Human Netrin 1 ELISA Kit | MyBioSource MBS725887 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Netrin 1 ELISA Kit

catalog :

MBS725887

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

citations: 1

Reference
Lewandowska L, Matuszkiewicz RowiÅ„ska J, Jayakumar C, Oldakowska Jedynak U, Looney S, Galas M, et al. Netrin-1 and semaphorin 3A predict the development of acute kidney injury in liver transplant patients. PLoS ONE. 2014;9:e107898 pubmed publisher

product information

catalog number :

MBS725887

products type :

ELISA Kit

products full name :

Human Netrin 1 ELISA Kit

products short name :

Netrin 1

other names :

netrin-1; Netrin-1; netrin-1; netrin 1, mouse, homolog of; epididymis tissue protein Li 131P; netrin 1; Epididymis tissue protein Li 131P

products gene name :

Ntn1

other gene names :

NTN1; NTN1; NTN1L; NTN1L

uniprot entry name :

NET1_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of Netrin-1. No significant cross-reactivity or interference between Netrin-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Netrin-1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive or Sandwich. Sensitivity: 1.0pg/mL.

products categories :

Neurobiology

products description :

Intended Uses This Netrin-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Netrin-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: Netrin-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Netrin-1. Standards or samples are then added to the microtiter plate wells and Netrin-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Netrin-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Netrin-1 are added to each well to "sandwich” the Netrin-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Netrin-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Netrin-1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

148613884

ncbi acc num :

NP_004813.2

ncbi gb acc num :

NM_004822.2

ncbi mol weight :

67,748 Da

ncbi pathways :

Alpha6-Beta4 Integrin Signaling Pathway (198807); Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); Cell-Cell Communication Pathway (477132); DCC Mediated Attractive Signaling Pathway (161000); DSCAM Interactions Pathway (187229); Developmental Biology Pathway (477129); Netrin Mediated Repulsion Signals Pathway (161001); Netrin-1 Signaling Pathway (160999)

ncbi summary :

Netrin is included in a family of laminin-related secreted proteins. The function of this gene has not yet been defined; however, netrin is thought to be involved in axon guidance and cell migration during development. Mutations and loss of expression of netrin suggest that variation in netrin may be involved in cancer development. [provided by RefSeq, Jul 2008]

uniprot summary :

NTN1: Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 17p13.1. Cellular Component: cytoplasm; extracellular region; basement membrane. Molecular Function: protein binding. Biological Process: axon guidance; cell-cell adhesion; inner ear morphogenesis; establishment of nucleus localization; apoptosis; positive regulation of axon extension; positive regulation of cell proliferation; neuron migration; negative regulation of axon extension; regulation of cell migration; anterior/posterior axon guidance

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage