catalog number :
MBS725886
products type :
ELISA Kit
products full name :
Guinea pig Inhibin ELISA Kit
products short name :
Inhibin
other names :
Inh; Inhibitor of prohead protease gp21; Inh; inhibitor of prohead protease gp21; Inhibitor of prohead protease gp21
other gene names :
inh; inh
uniprot entry name :
Q6U9C9_9CAUD
storage stability :
Store all reagents at 2-8 degree C.
products categories :
Neurobiology
products description :
Intended Uses: This INH ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofINHin the sample, thisINHELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusINHconcentration. The concentration ofINHin the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This INHenzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for INH. Standards or samples are then added to the microtiter plate wells andINHif present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofINHpresent in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forINHare added to each well to "sandwich" theINHimmobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containINHand enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
ncbi acc num :
NP_932528.1
ncbi gb acc num :
NC_005135.1
ncbi mol weight :
27,919 Da