ELISA/assay

Human Cholecystokinin octapeptide ELISA Kit

MBS725860

48-Strip-Wells

470 USD

ELISA Kit

Human Cholecystokinin octapeptide ELISA Kit

Cholecystokinin octapeptide

cholecystokinin preproprotein; Cholecystokinin; cholecystokinin; prepro-cholecystokinin; cholecystokinin triacontatriapeptide; cholecystokinin; (1-49)-CCK58

CCK 8

CCK; CCK; CCK; CCK58; CCK39; CCK33; CCK25; CCK18; CCK12; CCK8; CCK7; CCK5

CCKN_HUMAN

Human

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This CCK-8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CCK-8. This ELISA kit for research use only, not for therapeutic or diagnostic applications

Signal Transduction

Principle of the Assay: CCK-8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCK-8 antibody and an CCK-8-HRP conjugate. The assay sample and buffer are incubated together with CCK-8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCK-8 concentration since CCK-8 from samples and CCK-8-HRP conjugate compete for the anti-CCK-8 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCK-8 from the sample, fewer sites are left to bind CCK-8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCK-8 concentration in each sample is interpolated from this standard curve.

4502605

NP_000720.1

NM_000729.4

12,669 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

Cholecystokinin is a brain/gut peptide. In the gut, it induces the release of pancreatic enzymes and the contraction of the gallbladder. In the brain, its physiologic role is unclear. The cholecystokinin pro-hormone is processed by endo- and exo-proteolytic cleavages. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010]

CCK: This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion. Belongs to the gastrin/cholecystokinin family. Protein type: Hormone; Cell development/differentiation; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 3p22.1. Cellular Component: axon hillock; extracellular space; axon; dendrite; extracellular region; terminal button; perikaryon. Molecular Function: neuropeptide hormone activity; protein binding; hormone activity. Biological Process: caspase activation; release of cytochrome c from mitochondria; behavioral fear response; eating behavior; neuron migration; signal transduction; negative regulation of appetite; positive regulation of protein oligomerization; positive regulation of peptidyl-tyrosine phosphorylation; axonogenesis; positive regulation of mitochondrial depolarization; positive regulation of cell proliferation; protein kinase C activation; regulation of sensory perception of pain

48-Strip-Wells

470 USD

96-Strip-Wells

675