ELISA/assay

Rat a1-microglobulin ELISA Kit

MBS725834

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Rat a1-microglobulin ELISA Kit

a1-microglobulin

alpha-1-microglobulin; Protein AMBP; protein AMBP; bikunin; uristatin; protein HC; trypstatin; uronic-acid-rich protein; growth-inhibiting protein 19; inter-alpha-trypsin inhibitor light chain; complex-forming glycoprotein heterogeneous in charge; alpha-1-microglobulin/bikunin precursor; Alpha-1 microglycoprotein; Complex-forming glycoprotein heterogeneous in chargeInter-alpha-trypsin inhibitor light chain; ITI-LC; Alternative name(s):; Bikunin; EDC1; HI-30; Uronic-acid-rich protein

A1-MG

AMBP; AMBP; A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC; HCP; ITIL; Protein HC; ITI-LC

AMBP_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of alpha1MG. No significant cross-reactivity or interference between alpha1MG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between alpha1MG and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Cardiovascular

Intended Uses: This alpha1MG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat alpha1MG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay alpha1MG ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for alpha1MG. Standards or samples are then added to the microtiter plate wells and alpha1MG if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of alpha1MG present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for alpha1MG are added to each well to "sandwich" the alpha1MG immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain alpha1MG and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alpha1MG concentration in each sample is interpolated from this standard curve.

579676

CAA38587

P02760

38,999 Da

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Scavenging Of Heme From Plasma Pathway (771600)

This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq, Jul 2008]

Function: Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization. Ref.18Trypstatin is a trypsin inhibitor . By similarity. Ref.18. Subunit structure: I-alpha-I plasma protease inhibitors are assembled from one or two heavy chains (H1, H2 or H3) and one light chain, bikunin. Inter-alpha-inhibitor (I-alpha-I) is composed of H1, H2 and bikunin, inter-alpha-like inhibitor (I-alpha-LI) of H2 and bikunin, and pre-alpha-inhibitor (P-alpha-I) of H3 and bikunin. Alpha-1-microglobulin occurs as a monomer and also in complexes with IgA and albumin. Alpha-1-microglobulin interacts with FN1. Trypstatin is a monomer and also occurs as a complex with tryptase in mast cells . By similarity. Alpha-1-microglobulin and bikunin interact (via SH3 domain) with HEV ORF3 protein. Ref.26 Ref.27. Subcellular location: Secreted. Tissue specificity: Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine. Post-translational modification: The precursor is proteolytically processed into separately functioning proteins.3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate . By similarity.N- and O-glycosylated. N-glycan heterogeneity at Asn-115: Hex5HexNAc4 (major), Hex6HexNAc5 (minor) and dHex1Hex6HexNAc5 (minor). N-glycan at Asn-250: Hex5HexNAc4. O-linkage of the glycosaminoglycan, chondroitin sulfate, at Ser-215 allows cross-linking between the three polypeptide chains. Ref.16 Ref.17 Ref.20 Ref.22 Ref.28. Miscellaneous: In vitro, the first twelve residues of the amino end of the inhibitor appear to have a reactive site capable of inhibiting the activity of a number of enzymes. Its in vivo function is not known. Sequence similarities: In the N-terminal section; belongs to the calycin superfamily. Lipocalin family.Contains 2 BPTI/Kunitz inhibitor domains.

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675