ELISA/assay

Mouse Aquaporin 4 ELISA Kit

MBS725819

48-Strip-Wells

470 USD

ELISA Kit

Mouse Aquaporin 4 ELISA Kit

Aquaporin 4

aquaporin-4 isoform b; Aquaporin-4; aquaporin-4; WCH4; aquaporin type4; aquaporin 4 isoform delta4; mercurial-insensitive water channel; aquaporin 4; Mercurial-insensitive water channel; MIWC; WCH4

AQP4

AQP4; AQP4; MIWC; HMIWC2; AQP-4; MIWC

AQP4_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This AQP-4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse AQP-4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the Assay: AQP-4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AQP-4 antibody and an AQP-4-HRP conjugate. The assay sample and buffer are incubated together with AQP-4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AQP-4 concentration since AQP-4 from samples and AQP-4-HRP conjugate compete for the anti-AQP-4 antibody binding site. Since the number of sites is limited, as more sites are occupied by AQP-4 from the sample, fewer sites are left to bind AQP-4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AQP-4 concentration in each sample is interpolated from this standard curve.

4755125

NP_004019.1

NM_004028.3

32,300 Da

Aquaporin-mediated Transport Pathway (187187); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Passive Transport By Aquaporins Pathway (187188); SIDS Susceptibility Pathways (198901); Spinal Cord Injury Pathway (739007); Transmembrane Transport Of Small Molecules Pathway (106572); Vasopressin Regulates Renal Water Homeostasis Via Aquaporins Pathway (187189); Vasopressin-regulated Water Reabsorption Pathway (143700); Vasopressin-regulated Water Reabsorption Pathway (143631)

This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

AQP4: a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The predominant aquaporin found in brain. Two alternatively spliced transcript variants have been described. Protein type: Transporter, aquaporin family; Transporter; Membrane protein, integral; Channel, misc.; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 18q11.2-q12.1. Cellular Component: protein complex; integral to plasma membrane; basolateral plasma membrane; cytoplasm; T-tubule; plasma membrane; intercellular junction; external side of plasma membrane. Molecular Function: water transporter activity; water channel activity; porin activity; glycerol channel activity. Biological Process: glycerol transport; cellular water homeostasis; water transport; carbon dioxide transport; response to glucocorticoid stimulus; female pregnancy; response to radiation; sensory perception of sound; hyperosmotic salinity response; transport; renal water homeostasis; multicellular organismal water homeostasis; transmembrane transport; protein homooligomerization

48-Strip-Wells

470 USD

96-Strip-Wells

675