ELISA/assay

Mouse Anti-Teichoic acid antibody ELISA Kit

MBS725816

48-Strip-Wells

470 USD

ELISA Kit

Mouse Anti-Teichoic acid antibody ELISA Kit

Anti-Teichoic acid antibody

TA

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 ng/mL.

Intended Uses: This ATAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ATAb. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

For Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma. Intended Uses: This ATAAB ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of ATAAB in the sample, this ATAAB ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus ATAAB concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of ATAAB utilizes a multiclonal anti-ATAAB antibody and an ATAAB-HRP conjugate. The assay sample and buffer are incubated together with ATAAB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATAAB concentration since ATAAB from samples and ATAAB-HRP conjugate compete for the anti-ATAAB antibody binding site. Since the number of sites is limited, as more sites are occupied by ATAAB from the sample, fewer sites are left to bind ATAAB-HRP conjugate. Standards of known ATAAB concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of ATAAB. The ATAAB concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470 USD

96-Strip-Wells

675