Rat Syndecan 4 ELISA Kit | MyBioSource MBS725806 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Syndecan 4 ELISA Kit

catalog :

MBS725806

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS725806

products type :

ELISA Kit

products full name :

Rat Syndecan 4 ELISA Kit

products short name :

Syndecan 4

other names :

syndecan-4; Syndecan-4; syndecan-4; amphiglycan; ryudocan amphiglycan; ryudocan core protein; syndecan proteoglycan 4; syndecan 4 (amphiglycan, ryudocan); syndecan 4; Amphiglycan; Ryudocan core protein

products gene name :

SDC4

other gene names :

SDC4; SDC4; SYND4; SYND4

uniprot entry name :

SDC4_HUMAN

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of SDC-4. No significant cross-reactivity or interference between SDC-4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SDC-4 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This SDC-4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat SDC-4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cell Biology

products description :

Principle of the assay: SDC-4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SDC-4. Standards or samples are then added to the microtiter plate wells and SDC-4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SDC-4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SDC-4 are added to each well to "sandwich" the SDC-4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SDC-4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDC-4 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

38201675

ncbi acc num :

NP_002990.2

ncbi gb acc num :

NM_002999.3

ncbi mol weight :

16,545 Da

ncbi pathways :

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (645305); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Glycogen Storage Diseases Pathway (980468)

ncbi summary :

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. The encoded protein is found as a homodimer and is a member of the syndecan proteoglycan family. This gene is found on chromosome 20, while a pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]

uniprot summary :

syndecan-4: a heparan sulfate proteoglycan type I membrane protein that belongs to the syndecan proteoglycan family. Expressed as a homodimer. Promotes cell spreading in a beta(1) integrin-dependent fashion through PKC-alpha and Rho. Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 20q12. Cellular Component: lysosomal lumen; costamere; cell surface; focal adhesion; Golgi lumen; integral to plasma membrane; plasma membrane; lipid raft. Molecular Function: protein binding; protein kinase C binding; fibronectin binding. Biological Process: chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; phototransduction, visible light; extracellular matrix organization and biogenesis; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; positive regulation of focal adhesion formation; ureteric bud development; positive regulation of protein kinase activity; positive regulation of stress fiber formation; carbohydrate metabolic process; pathogenesis; retinoid metabolic process

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage