ELISA/assay

Mouse Hepatocyte Nuclear Factor 4 Alpha ELISA Kit

MBS725804

48-Strip-Wells

470 USD

ELISA Kit

Mouse Hepatocyte Nuclear Factor 4 Alpha ELISA Kit

Hepatocyte Nuclear Factor 4 Alpha

hepatocyte nuclear factor 4-alpha isoform HNF4alpha7; Hepatocyte nuclear factor 4-alpha; hepatocyte nuclear factor 4-alpha; TCF-14; HNF4alpha10/11/12; transcription factor 14; transcription factor HNF-4; hepatic nuclear factor 4 alpha; nuclear receptor subfamily 2 group A member 1; hepatocyte nuclear factor 4, alpha; Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4

HNF4alpha

HNF4A; HNF4A; TCF; HNF4; MODY; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha; HNF4; NR2A1; TCF14; HNF-4-alpha; TCF-14

HNF4A_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich

Epigenetics and Nuclear Signaling

For Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This HNF4alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HNF4alpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: HNF4alpha ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HNF4alpha. Standards or samples are then added to the microtiter plate wells and HNF4alpha if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HNF4alpha present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HNF4alpha are added to each well to "sandwich" the HNF4alpha immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HNF4alpha and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HNF4alpha concentration in each sample is interpolated from this standard curve.

71725341

NP_001025174.1

NM_001030003.2

43,988 Da

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Developmental Biology Pathway (477129); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); HIF-1-alpha Transcription Factor Network Pathway (138045); Integrated Pancreatic Cancer Pathway (711360); Maturity Onset Diabetes Of The Young Pathway (83096)

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]

HNF4 alpha: a nuclear transcription factor which binds DNA as a homodimer. Controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic gene, alpha 1-antitrypsin, apolipoprotein CIII, and transthyretin genes. May be essential for development of the liver, kidney and intestine. Mutations have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Four alternatively spliced isoforms have been described. Protein type: DNA-binding; Transcription factor; Nuclear receptor. Chromosomal Location of Human Ortholog: 20q13.12. Cellular Component: nucleoplasm; cytoplasm; nucleus. Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; ligand-dependent nuclear receptor activity; protein homodimerization activity; DNA binding; zinc ion binding; steroid hormone receptor activity; transcription factor activity; fatty acid binding; receptor binding. Biological Process: transcription initiation from RNA polymerase II promoter; regulation of lipid metabolic process; intracellular receptor-mediated signaling pathway; positive regulation of transcription, DNA-dependent; lipid homeostasis; glucose homeostasis; endocrine pancreas development; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; regulation of gastrulation; xenobiotic metabolic process; response to glucose stimulus; ornithine metabolic process; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; lipid metabolic process; negative regulation of cell growth; blood coagulation; regulation of insulin secretion; sex differentiation; phospholipid homeostasis. Disease: Fanconi Renotubular Syndrome 4 With Maturity-onset Diabetes Of The Young; Maturity-onset Diabetes Of The Young, Type 1; Diabetes Mellitus, Noninsulin-dependent

48-Strip-Wells

470 USD

96-Strip-Wells

675