Human Bone Morphogenetic Protein 1 ELISA Kit | MyBioSource MBS725794 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Bone Morphogenetic Protein 1 ELISA Kit

catalog :

MBS725794

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS725794

products type :

ELISA Kit

products full name :

Human Bone Morphogenetic Protein 1 ELISA Kit

products short name :

Bone Morphogenetic Protein 1

other names :

bone morphogenetic protein 1 isoform 3; Bone morphogenetic protein 1; bone morphogenetic protein 1; mammalian tolloid protein; procollagen C-proteinase 3; procollagen C-endopeptidase; bone morphogenetic protein 1; Mammalian tolloid protein; mTld; Procollagen C-proteinase; PCP

products gene name :

BMP1

other gene names :

BMP1; BMP1; PCP; TLD; OI13; PCP2; PCOLC; PCOLC; BMP-1; mTld; PCP

uniprot entry name :

BMP1_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of BMP-1. No significant cross-reactivity or interference between BMP-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BMP-1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive or Sandwich. Sensitivity: 1.0pg/mL.

products categories :

Signal Transduction

products description :

Intended Uses: This BMP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BMP-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: BMP-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for BMP-1. Standards or samples are then added to the microtiter plate wells and BMP-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of BMP-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for BMP-1 are added to each well to “sandwich” the BMP-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain BMP-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMP-1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

5453579

ncbi acc num :

NP_006120.1

ncbi gb acc num :

NM_006129.4

ncbi mol weight :

92,655 Da

ncbi pathways :

Adipogenesis Pathway (198832); Anchoring Fibril Formation Pathway (730307); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Cardiac Progenitor Differentiation Pathway (712094); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); Crosslinking Of Collagen Fibrils Pathway (730308); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); HDL-mediated Lipid Transport Pathway (106158)

ncbi summary :

This gene encodes a protein that is capable of inducing formation of cartilage in vivo. Although other bone morphogenetic proteins are members of the TGF-beta superfamily, this gene encodes a protein that is not closely related to other known growth factors. This gene is expressed as alternatively spliced variants that share an N-terminal protease domain but differ in their C-terminal region. [provided by RefSeq, Aug 2008]

uniprot summary :

BMP1: Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD). Defects in BMP1 are a cause of autosomal recessive osteogenesis imperfecta (AR-OI). A connective tissue disorder characterized by bone fragility, progressively deforming bones, bowing of limbs due to multiple fractures, very short stature, a triangular face, severe scoliosis, and grayish sclera. AR-OI due to BMP1 mutations belongs to the group of osteogenesis imperfecta type III in the Sillence classification. Belongs to the peptidase M12A family. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Protease; Cytokine; EC 3.4.24.19. Chromosomal Location of Human Ortholog: 8p21.3. Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; extracellular space; extracellular region. Molecular Function: peptidase activity; growth factor activity; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; cytokine activity; calcium ion binding. Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; ossification; multicellular organismal development; lipoprotein metabolic process; cell differentiation; proteolysis; skeletal development; cartilage condensation. Disease: Osteogenesis Imperfecta, Type Xiii

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage