Human Hormone Sensitive Lipase ELISA Kit | MyBioSource MBS725789 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Hormone Sensitive Lipase ELISA Kit

catalog :

MBS725789

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS725789

products type :

ELISA Kit

products full name :

Human Hormone Sensitive Lipase ELISA Kit

products short name :

Hormone Sensitive Lipase

other names :

hormone-sensitive lipase; Hormone-sensitive lipase; hormone-sensitive lipase; hormone-sensitive lipase testicular isoform; lipase, hormone-sensitive

products gene name :

HSL

other gene names :

LIPE; LIPE; HSL; LHS; AOMS4; HSL

uniprot entry name :

LIPS_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of HSL. No significant cross-reactivity or interference between HSL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSL and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This HSL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HSL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cardiovascular

products description :

. Principle of the assay: HSL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HSL antibody and an HSL-HRP conjugate. The assay sample and buffer are incubated together with HSL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSL concentration since HSL from samples and HSL-HRP conjugate compete for the anti-HSL antibody binding site. Since the number of sites is limited, as more sites are occupied by HSL from the sample, fewer sites are left to bind HSL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSL concentration in each sample is interpolated from this standard curve.

ncbi gi num :

21328446

ncbi acc num :

NP_005348.2

ncbi gb acc num :

NM_005357.3

ncbi mol weight :

84,128 Da

ncbi pathways :

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adipogenesis Pathway (198832); Fatty Acid Beta Oxidation Pathway (198865); Hormone-sensitive Lipase (HSL)-mediated Triacylglycerol Hydrolysis Pathway (106121); Insulin Signaling Pathway (198845); Insulin Signaling Pathway (83090); Insulin Signaling Pathway (501); Lipid Digestion, Mobilization, And Transport Pathway (106111)

ncbi summary :

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008]

uniprot summary :

HSL: hormone sensitive lipase is a lipolytic enzyme of the GDXG family. Plays a rate limiting step in triglyceride lipolysis. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin. Protein type: Lipase; EC 3.1.1.79. Chromosomal Location of Human Ortholog: 19q13.2. Cellular Component: lipid particle; caveola; cytosol. Molecular Function: hormone-sensitive lipase activity; triacylglycerol lipase activity; protein binding; protein kinase binding. Biological Process: cholesterol metabolic process; triacylglycerol catabolic process; diacylglycerol catabolic process; protein amino acid phosphorylation; lipid catabolic process; long-chain fatty acid catabolic process. Disease: Abdominal Obesity-metabolic Syndrome 4

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage