ELISA/assay

Mouse Hyaluronidase ELISA Kit

MBS725780

48-Strip-Wells

470 USD

ELISA Kit

Mouse Hyaluronidase ELISA Kit

Hyaluronidase

hyaluronidase; Hyaluronidase-3; hyaluronidase-3; lung carcinoma protein 3; hyaluronoglucosaminidase 3; Hyaluronoglucosaminidase-3; Lung carcinoma protein 3; LuCa-3

HAase

HYAL3; HYAL3; LUCA3; HYAL-3; LUCA-3; LUCA3; Hyal-3; LuCa-3

HYAL3_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 U/L.

Signal Transduction

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This HAD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HAD. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY HAD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HAD antibody and an HAD-HRP conjugate. The assay sample and buffer are incubated together with HAD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HAD concentration since HAD from samples and HAD-HRP conjugate compete for the anti-HAD antibody binding site. Since the number of sites is limited, as more sites are occupied by HAD from the sample, fewer sites are left to bind HAD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HAD concentration in each sample is interpolated from this standard curve.

2935328

AAC70915.1

15,865 Da

CS/DS Degradation Pathway (645311); Chondroitin Sulfate Degradation Pathway (413376); Chondroitin Sulfate Degradation Pathway (468247); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Dermatan Sulfate Degradation Pathway (413375); Dermatan Sulfate Degradation Pathway (468240); Disease Pathway (530764); Glycogen Storage Diseases Pathway (980468); Glycosaminoglycan Degradation Pathway (82981); Glycosaminoglycan Degradation Pathway (355)

This gene encodes a member of the hyaluronidase family. Hyaluronidases are endoglycosidase enzymes that degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. The regulated turnover of hyaluronan plays a critical role in many biological processes including cell proliferation, migration and differentiation. The encoded protein may also play an important role in sperm function. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression, and the expression of specific transcript variants may be indicative of tumor status. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and some isoforms may lack hyaluronidase activity. This gene overlaps and is on the same strand as N-acetyltransferase 6 (GCN5-related), and some transcripts of each gene share a portion of the first exon. [provided by RefSeq, Jan 2011]

HYAL3: a member of the hyaluronidase family. Hyaluronidases are endoglycosidase enzymes that degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. The regulated turnover of hyaluronan plays a critical role in many biological processes including cell proliferation, migration and differentiation. The encoded protein may also play an important role in sperm function. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression, and the expression of specific transcript variants may be indicative of tumor status. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and some isoforms may lack hyaluronidase activity. This gene overlaps and is on the same strand as N-acetyltransferase 6 (GCN5-related), and some transcripts of each gene share a portion of the first exon. [provided by RefSeq, Jan 2011]. Protein type: Secreted, signal peptide; EC 3.2.1.35; Secreted; Hydrolase. Chromosomal Location of Human Ortholog: 3p21.3. Cellular Component: lysosome; extracellular region; cytoplasmic vesicle. Molecular Function: viral receptor activity; hyaluronoglucuronidase activity; hyalurononglucosaminidase activity. Biological Process: response to antibiotic; response to virus; cartilage development; carbohydrate metabolic process; inflammatory response; hyaluronan catabolic process

48-Strip-Wells

470 USD

96-Strip-Wells

675