Mouse Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 ELISA Kit | MyBioSource MBS725764 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 ELISA Kit

catalog :

MBS725764

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS725764

products type :

ELISA Kit

products full name :

Mouse Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 ELISA Kit

products short name :

Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4

other names :

NADPH oxidase 4 isoform c; NADPH oxidase 4; NADPH oxidase 4; kidney oxidase-1; renal NAD(P)H-oxidase; kidney superoxide-producing NADPH oxidase; NADPH oxidase 4; Kidney oxidase-1; KOX-1; Kidney superoxide-producing NADPH oxidase; Renal NAD(P)H-oxidase

products gene name :

NOX4

other gene names :

NOX4; NOX4; KOX; KOX-1; RENOX; RENOX; KOX-1

uniprot entry name :

NOX4_HUMAN

reactivity :

Mouse

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This NOX4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse NOX4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the Assay: NOX4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NOX4 antibody and an NOX4 -HRP conjugate. The assay sample and buffer are incubated together with NOX4 -HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NOX4 concentration since NOX4 from samples and NOX4 -HRP conjugate compete for the anti-NOX4 antibody binding site. Since the number of sites is limited, as more sites are occupied by NOX4 from the sample, fewer sites are left to bind NOX4 -HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NOX4 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

219842348

ncbi acc num :

NP_001137309.1

ncbi gb acc num :

NM_001143837.1

ncbi mol weight :

59,915 Da

ncbi pathways :

Oxidative Stress Pathway (198916); Signaling Events Mediated By PTP1B Pathway (138017); Spinal Cord Injury Pathway (739007)

ncbi summary :

This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]

uniprot summary :

NOX4: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Oxidoreductase; Membrane protein, multi-pass; Cell cycle regulation; Membrane protein, integral; Nucleolus; EC 1.6.3.-. Chromosomal Location of Human Ortholog: 11q14.2-q21. Cellular Component: endoplasmic reticulum membrane; focal adhesion; mitochondrion; perinuclear region of cytoplasm; apical plasma membrane; integral to membrane; nucleolus; stress fiber; NADPH oxidase complex. Molecular Function: FAD binding; oxygen sensor activity; electron carrier activity; NAD(P)H oxidase activity; superoxide-generating NADPH oxidase activity; heme binding; nucleotide binding. Biological Process: positive regulation of apoptosis; cell morphogenesis; cell aging; homocysteine metabolic process; positive regulation of smooth muscle cell migration; cardiac muscle cell differentiation; negative regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of MAP kinase activity; positive regulation of stress fiber formation; response to hypoxia; inflammatory response; superoxide release; bone resorption

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage