Rat Soluble Fms-like tyrosine kinase Receptor 1 ELISA Kit | MyBioSource MBS725733 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Soluble Fms-like tyrosine kinase Receptor 1 ELISA Kit

catalog :

MBS725733

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS725733

products type :

ELISA Kit

products full name :

Rat Soluble Fms-like tyrosine kinase Receptor 1 ELISA Kit

products short name :

Soluble Fms-like tyrosine kinase Receptor 1

other names :

sFlt-1; Vascular endothelial growth factor receptor 1; vascular endothelial growth factor receptor 1; embryonic receptor kinase 2; tyrosine-protein kinase receptor FLT; vascular permeability factor receptor; vascular endothelial growth factor receptor-1; FMS-like tyrosine kinase 1; Embryonic receptor kinase 2; Fms-like tyrosine kinase 1; FLT-1; Tyrosine-protein kinase receptor FLT

products gene name :

sFLt-1

other gene names :

Flt1; Flt1; Flt-1; sFlt1; VEGFR1; VEGFR-1; AI323757; Emrk2; Flt; Vegfr1; VEGFR-1; FLT-1

uniprot entry name :

VGFR1_MOUSE

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of sFLTR-1. No significant cross-reactivity or interference between sFLTR-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sFLTR-1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

products description :

Intended Uses This sFLTR-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat sFLTR-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: sFLTR-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-sFLTR-1 antibody and an sFLTR-1-HRP conjugate. The assay sample and buffer are incubated together with sFLTR-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sFLTR-1 concentration since sFLTR-1 from samples and sFLTR-1-HRP conjugate compete for the anti-sFLTR-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by sFLTR-1 from the sample, fewer sites are left to bind sFLTR-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sFLTR-1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

2809071

ncbi acc num :

BAA24499.1

ncbi mol weight :

149,876 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Endocytosis Pathway (102286); Endocytosis Pathway (102181); Focal Adhesion Pathway (198353); Focal Adhesion Pathway (83264); Focal Adhesion Pathway (478); HIF-1 Signaling Pathway (695223); Id Signaling Pathway (198411); Neurophilin Interactions With VEGF And VEGFR Pathway (1000920)

uniprot summary :

VEGFR1: a receptor tyrosine kinase of the VEGFR family. Receptor for VEGF, VEGFB and PGF. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Two splice variant isoforms have been described. Isoform SFlt1 may have an inhibitory role in angiogenesis. Protein type: Kinase, protein; Membrane protein, integral; Protein kinase, TK; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; VEGFR family. Cellular Component: focal adhesion; membrane; integral to plasma membrane; plasma membrane; integral to membrane; endosome; receptor complex. Molecular Function: transferase activity; identical protein binding; vascular endothelial growth factor receptor activity; growth factor binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity. Biological Process: cell migration; peptidyl-tyrosine phosphorylation; multicellular organismal development; protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase activity; chemotaxis; protein amino acid phosphorylation; positive regulation of vascular endothelial growth factor receptor signaling pathway; patterning of blood vessels; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of MAP kinase activity; monocyte chemotaxis; positive regulation of angiogenesis; positive regulation of MAPKKK cascade; response to hypoxia; blood vessel morphogenesis; embryonic morphogenesis; angiogenesis; sprouting angiogenesis; cell differentiation; vascular endothelial growth factor receptor signaling pathway; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage