ELISA/assay

Human Arrestin beta 1 ELISA Kit

MBS725731

48-Strip-Wells

440 USD

ELISA Kit

Human Arrestin beta 1 ELISA Kit

[Arrestin beta 1]

[Human Arrestin b 1 ELISA Kit]

[arrestin beta 1 transcript variant 2; Beta-arrestin-1; beta-arrestin-1; arrestin 2; arrestin, beta 1; Arrestin beta-1]

[ARRb1]

[ARRb1]

[ARRB1; ARRB1; ARB1; ARR1; ARR1]

ARRB1_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of ARRbeta1. No significant cross-reactivity or interference between ARRbeta1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ARRbeta1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Detection Range: 0.5-10ng/mL. Sensitivity: 0.1ng/mL

Signal Transduction

Intended Uses: This ARRbeta1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ARRbeta1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ARRbeta1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ARRbeta1 antibody and an ARRbeta 1-HRP conjugate. The assay sample and buffer are incubated together with ARRbeta1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARRbeta1 concentration since ARRbeta1 from samples and ARRbeta 1-HRP conjugate compete for the anti-ARRbeta1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ARRbeta1 from the sample, fewer sites are left to bind ARRbeta1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARRbeta1 concentration in each sample is interpolated from this standard curve.

209944101

ACI96306.1

46,309 Da

Activated NOTCH1 Transmits Signal To The Nucleus Pathway (576270); CXCR3-mediated Signaling Events Pathway (138011); Calcium Regulation In The Cardiac Cell Pathway (198906); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Clathrin Derived Vesicle Budding Pathway (119545); Corticotropin-releasing Hormone Pathway (920957); Disease Pathway (530764); Endocytosis Pathway (102279); Endocytosis Pathway (102181)

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described. [provided by RefSeq, Jan 2011]

ARRB1: regulates G-protein coupled receptors (GPCR) signaling by mediating both receptor desensitization and resensitization processes. Binds to GRK-phosphorylated receptor and sterically preclude its coupling to the cognate G- protein; the binding appears to require receptor determinants exposed only in the active receptor conformation. Targets many receptors for internalization by acting as endocytic adapters (CLASPs, clathrin-associated sorting proteins). Internalized arrestin-receptor complexes traffic to intracellular endosomes, where they remain uncoupled from G-proteins. Two different modes of arrestin-mediated internalization occur. Beta-arrestins function as multivalent adapter proteins that can switch the GPCR from a G-protein signaling mode that transmits short-lived signals from the plasma membrane via small molecule second messengers and ion channels to a beta-arrestin signaling mode that transmits a distinct set of signals that are initiated as the receptor internalizes and transits the intracellular compartment. Also involved in regulation of receptors other than GPCRs. Involved in Toll-like receptor and IL-1 receptor signaling through the interaction with TRAF6 which prevents TRAF6 autoubiquitination and oligomerization required for activation of NF-kappa-B and JUN. Binds phosphoinositides. Binds inositolhexakisphosphate (InsP6). Involved in IL8-mediated granule release in neutrophils. Interacts with phosphorylated ADRB2 and CHRM2. Interacts with SRC (via the SH3 domain and the protein kinase domain); the interaction is independent of the phosphorylation state of SRC C-terminus. Interacts with RAF1, CHUK, IKBKB and Nik. Interacts with DVL1 and DVL2; the interaction is enhanced by DVL phosphorylation. Interacts with IGF1R. Belongs to the arrestin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: cytoplasmic vesicle membrane; lysosomal membrane; coated pit; pseudopodium; cytosol; nucleoplasm; Golgi membrane; cytoplasm; plasma membrane; heterotrimeric G-protein complex; cytoplasmic vesicle; nucleus; chromatin. Molecular Function: enzyme inhibitor activity; angiotensin receptor binding; insulin-like growth factor receptor binding; protein binding; histone acetyltransferase activity; mitogen-activated protein kinase kinase binding; ubiquitin protein ligase binding; caspase inhibitor activity; transcription factor binding; GTPase activator activity. Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; platelet activation; Notch signaling pathway; positive regulation of protein binding; positive regulation of receptor internalization; positive regulation of Rho protein signal transduction; protein ubiquitination; negative regulation of caspase activity; positive regulation of peptidyl-serine phosphorylation; protein transport; G-protein coupled receptor internalization; inhibition of NF-kappaB transcription factor; stress fiber formation; phototransduction; negative regulation of interleukin-8 production; negative regulation of interleukin-6 production; negative regulation of protein ubiquitination; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; post-Golgi vesicle-mediated transport; positive regulation of protein amino acid phosphorylation; blood coagulation; positive regulation of GTPase activity

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415