product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human 20S proteasome ELISA Kit
catalog :
MBS725729
quantity :
48-Strip-Wells
price :
440 USD
more info or order :
image
image 1 :
product information
catalog number :
MBS725729
products type :
ELISA Kit
products full name :
Human 20S proteasome ELISA Kit
products short name :
[20S proteasome]
other names :
[20S proteasome; Proteasome subunit beta type; 20S proteasome]
products gene name :
[20SP]
other gene names :
[NAEGRDRAFT_77167]
uniprot entry name :
D2UXF6_NAEGR
reactivity :
Human
specificity :
This assay has high sensitivity and excellent specificity for detection of 20S-PSM. No significant cross-reactivity or interference between 20S-PSM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between 20S-PSM and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 0.5-10ng/mL. Sensitivity: 0.1ng/mL
products categories :
Cell Biology
products description :
Intended Uses: This 20S-PSM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human 20S-PSM. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: 20S-PSM ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-20S-PSM antibody and an 20S-PSM-HRP conjugate. The assay sample and buffer are incubated together with 20S-PSM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 20S-PSM concentration since 20S-PSM from samples and 20S-PSM-HRP conjugate compete for the anti-20S-PSM antibody binding site. Since the number of sites is limited, as more sites are occupied by 20S-PSM from the sample, fewer sites are left to bind 20S-PSM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 20S-PSM concentration in each sample is interpolated from this standard curve.
ncbi gi num :
284096649
ncbi acc num :
EFC50276.1
ncbi mol weight :
27,433 Da
ncbi pathways :
Proteasome Pathway (151521); Proteasome Pathway (445); Proteasome, 20S Core Particle Pathway (424442); Proteasome, 20S Core Particle Pathway (890592)
uniprot summary :
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
size1 :
48-Strip-Wells
price1 :
440 USD
size2 :
96-Strip-Wells
price2 :
640
size3 :
5x96-Strip-Wells
price3 :
2895
size4 :
10x96-Strip-Wells
price4 :
5415
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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