ELISA/assay

Mouse heart fatty acid binding protein, h-FABP ELISA Kit

MBS725708

48-Strip-Wells

470 USD

ELISA Kit

Mouse heart fatty acid binding protein, h-FABP ELISA Kit

heart fatty acid binding protein, h-FABP

Mouse heart fatty acid binding protein ELISA Kit; Mouse heart fatty acid binding protein, h-FABP ELISA Kit; heart fatty acid binding protein, h-FABP; heart fatty acid binding protein, h-FABP ELISA Kit (Mouse)

heart fatty acid binding protein; Fatty acid-binding protein, heart; fatty acid-binding protein, heart; M-FABP; OTTHUMP00000003898; fatty acid binding protein 11; mammary-derived growth inhibitor; muscle fatty acid-binding protein; Fatty acid-binding protein 3, muscle; heart-type fatty acid-binding protein; fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor); Fatty acid-binding protein 3; Heart-type fatty acid-binding protein; H-FABP; Mammary-derived growth inhibitor; MDGI; Muscle fatty acid-binding protein

H-FABP

FABP3; FABP3; MDGI; FABP11; H-FABP; O-FABP; FABP11; MDGI

FABPH_HUMAN

Mouse

Store all reagents at 2-8 degree C

Assay Type: Sandwich

Mouse ELISA Kit

Intended Uses: This H-FABP ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofH-FABP in the sample, thisH-FABP ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusH-FABP concentration. The concentration ofH-FABP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This H-FABP enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forH-FABP. Standards or samples are then added to the microtiter plate wells andH-FABP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofH-FABP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forH-FABP are added to each well to "sandwich" theH-FABP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containH-FABP and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

458862

P05413

14,858 Da

PPAR Signaling Pathway (83042); PPAR Signaling Pathway (450)

The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer. [provided by RefSeq]

Function: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. Subcellular location: Cytoplasm. Domain: Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. Sequence similarities: Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

48-Strip-Wells

470 USD

96-Strip-Wells

675