Mouse Perforin 1 ELISA Kit | MyBioSource MBS725661 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Perforin 1 ELISA Kit

catalog :

MBS725661

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS725661

products type :

ELISA Kit

products full name :

Mouse Perforin 1 ELISA Kit

products short name :

Perforin 1

other names :

perforin-1; Perforin-1; perforin-1; cytolysin; lymphocyte pore forming protein; lymphocyte pore-forming protein; perforin 1 (pore forming protein); Cytolysin; Lymphocyte pore-forming protein; PFP

products gene name :

PRF1

other gene names :

PRF1; PRF1; P1; PFP; FLH2; PFN1; HPLH2; PFP; P1; PFP

uniprot entry name :

PERF_HUMAN

reactivity :

Mouse

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This PRF1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PRF1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Immunology

products description :

Principle of the Assay: PRF1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PRF1 antibody and an PRF1-HRP conjugate. The assay sample and buffer are incubated together with PRF1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRF1 concentration since PRF1 from samples and PRF1-HRP conjugate compete for the anti-PRF1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PRF1 from the sample, fewer sites are left to bind PRF1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRF1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

133908621

ncbi acc num :

NP_001076585.1

ncbi gb acc num :

NM_001083116.1

ncbi mol weight :

61,377 Da

ncbi pathways :

Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Apoptosis Pathway (198797); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Caspase Cascade In Apoptosis Pathway (137974); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Graft-versus-host Disease Pathway (83124); Graft-versus-host Disease Pathway (536)

ncbi summary :

The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

uniprot summary :

PRF1: Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. Monomer, as sobluble protein. Homooligomer. Oligomerization is required for pore formation. Repressed by contact with target cells. Belongs to the complement C6/C7/C8/C9 family. Protein type: Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 10q22. Cellular Component: membrane; cytoplasmic membrane-bound vesicle; extracellular region; plasma membrane; integral to membrane. Molecular Function: protein binding; calcium ion binding; wide pore channel activity. Biological Process: formation of immunological synapse; apoptosis; cytolysis; defense response to tumor cell; cellular defense response; immune response to tumor cell; defense response to virus; transmembrane transport; protein homooligomerization. Disease: Lymphoma, Non-hodgkin, Familial; Aplastic Anemia; Hemophagocytic Lymphohistiocytosis, Familial, 2

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage