ELISA/assay

Mouse Signal Transducer And Activator Of Transcription 6 ELISA Kit

MBS725637

48-Strip-Wells

470 USD

ELISA Kit

Mouse Signal Transducer And Activator Of Transcription 6 ELISA Kit

Signal Transducer And Activator Of Transcription 6

signal transducer and activator of transcription 6 isoform 1; Signal transducer and activator of transcription 6; signal transducer and activator of transcription 6; STAT, interleukin4-induced; transcription factor IL-4 STAT; signal transducer and activator of transcription 6, interleukin-4 induced; IL-4 Stat

STAT6

STAT6; STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT

STAT6_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of STAT6. No significant cross-reactivity or interference between STAT6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between STAT6 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Signal Transduction

Intended Uses: This STAT6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse STAT6. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay STAT6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-STAT6 antibody and an STAT6-HRP conjugate. The assay sample and buffer are incubated together with STAT6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the STAT6 concentration since STAT6 from samples and STAT6-HRP conjugate compete for the anti-STAT6 antibody binding site. Since the number of sites is limited, as more sites are occupied by STAT6 from the sample, fewer sites are left to bind STAT6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STAT6 concentration in each sample is interpolated from this standard curve.

23397678

NP_003144.3

NM_003153.4

81,748 Da

Adipogenesis Pathway (198832); Cytosolic Sensors Of Pathogen-associated DNA Pathway (576255); Downstream Signal Transduction Pathway (106385); Hepatitis B Pathway (694606); IL-3 Signaling Pathway (198881); IL-4 Signaling Pathway (198760); IL12-mediated Signaling Events Pathway (137922); IL4-mediated Signaling Events Pathway (137933); Immune System Pathway (106386); Inflammatory Bowel Disease (IBD) Pathway (842771)

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

STAT6: transcription factor of the STAT family. Plays a central role in IL4-mediated biological responses. Induces the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. May function in the differentiation of T helper 2 cells and class switch of immunoglobulins. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 12q13. Cellular Component: nucleoplasm; cytoplasm; nuclear chromatin; cytosol; lipid raft. Molecular Function: identical protein binding; signal transducer activity; protein binding; protein phosphatase binding; transcription factor activity. Biological Process: regulation of transcription from RNA polymerase II promoter; T-helper 1 cell lineage commitment; negative regulation of T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of interferon type I production; innate immune response; mammary gland epithelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of isotype switching to IgE isotypes; regulation of cell proliferation

48-Strip-Wells

470 USD

96-Strip-Wells

675