Human Vasoactive intestinal peptide ELISA Kit | MyBioSource MBS725630 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Vasoactive intestinal peptide ELISA Kit

catalog :

MBS725630

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS725630

products type :

ELISA Kit

products full name :

Human Vasoactive intestinal peptide ELISA Kit

products short name :

Vasoactive intestinal peptide

other names :

vasoactive intestinal peptide, partial; VIP peptides; VIP peptides; prepro-VIP; vasoactive intestinal peptide; Peptide histidine valine 42

products gene name :

VIP

other gene names :

VIP; VIP; PHM27; VIP

uniprot entry name :

VIP_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of VIP. No significant cross-reactivity or interference between VIP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between VIP and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive or Sandwich. Sensitivity: 1.0pg/mL.

products categories :

Cardiovascular

products description :

Intended Uses This VIP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human VIP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: VIP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for VIP. Standards or samples are then added to the microtiter plate wells and VIP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of VIP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for VIP are added to each well to "sandwich” the VIP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain VIP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VIP concentration in each sample is interpolated from this standard curve.

ncbi gi num :

340248

ncbi acc num :

AAA63268.1

ncbi mol weight :

19,082 Da

ncbi pathways :

G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

ncbi summary :

The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

uniprot summary :

VIP: VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder. Belongs to the glucagon family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 6q25. Cellular Component: cell soma; extracellular region. Molecular Function: neuropeptide hormone activity; protein binding; hormone activity. Biological Process: negative regulation of smooth muscle cell proliferation; mRNA stabilization; negative regulation of potassium ion transport; regulation of signal transduction; positive regulation of vasodilation; body fluid secretion; G-protein coupled receptor protein signaling pathway; regulation of protein localization; learning and/or memory; positive regulation of cell proliferation; positive regulation of protein catabolic process; positive regulation of endothelial cell proliferation; regulation of sensory perception of pain; negative regulation of apoptosis

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage