catalog number :
MBS725566
products type :
ELISA Kit
products full name :
Rat Total Adiponectin ELISA Kit
products short name :
Total Adiponectin
products gene name :
ADPN
specificity :
This assay has high sensitivity and excellent specificity for detection of APN. No significant cross-reactivity or interference between APN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between APN and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This APN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat APN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the assay: APN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for APN. Standards or samples are then added to the microtiter plate wells and APN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of APN present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for APN are added to each well to “sandwich” the APN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain APN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APN concentration in each sample is interpolated from this standard curve.
size1 :
48-Strip-Wells-(Competitive)
size2 :
48-Strip-Wells-(Sandwich)
size3 :
96-Strip-Wells-(Competitive)
size4 :
96-Strip-Wells-(Sandwich)