ELISA/assay

Human Extracellular Signal Regulated Kinase 2 ELISA Kit

MBS725544

48-Strip-Wells

470 USD

ELISA Kit

Human Extracellular Signal Regulated Kinase 2 ELISA Kit

Extracellular Signal Regulated Kinase 2

mitogen-activated protein kinase 1; mitogen-activated protein kinase 1; mitogen-activated protein kinase 2; extracellular signal-regulated kinase-2

ERK2

MAPK1; ERK2

Human

This assay has high sensitivity and excellent specificity for detection of ERK2. No significant cross-reactivity or interference between ERK2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ERK2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 ng/mL.

Intended Uses: This ERK2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ERK2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: ERK2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ERK2. Standards or samples are then added to the microtiter plate wells and ERK2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ERK2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ERK2 are added to each well to "sandwich" the ERK2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ERK2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ERK2 concentration in each sample is interpolated from this standard curve.

312062795

NP_001185851.1

NM_001198922.1

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48-Strip-Wells

470 USD

96-Strip-Wells

675