catalog number :
MBS725529
products type :
ELISA Kit
products full name :
Mouse Complement 4 ELISA Kit
products short name :
Complement 4
other names :
complement 4; complement 4; complement C4; MHC-linked complement C4; complement 4
other gene names :
C4; C4-2
specificity :
This assay has high sensitivity and excellent specificity for detection of C4. No significant cross-reactivity or interference between C4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C4 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 mg/dL.
products categories :
Immunology
products description :
Intended Uses This C4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse C4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: C4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C4 antibody and an C4-HRP conjugate. The assay sample and buffer are incubated together with C4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C4 concentration since C4 from samples and C4-HRP conjugate compete for the anti-C4 antibody binding site. Since the number of sites is limited, as more sites are occupied by C4 from the sample, fewer sites are left to bind C4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C4 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
NP_001070701.1
ncbi gb acc num :
NM_001077233.1