ELISA/assay

Guinea Pig oxidized lowdensity lipoprotein ELISA Kit

MBS725515

48-Strip-Wells

470 USD

ELISA Kit

Guinea Pig oxidized lowdensity lipoprotein ELISA Kit

oxidized lowdensity lipoprotein

Guinea Pig oxidized lowdensity lipoprotein ELISA Kit; oxidized lowdensity lipoprotein; oxidized lowdensity lipoprotein (Guinea Pig)

OxLDL

GuineaPig

This assay has high sensitivity and excellent specificity for detection of oxLDL. No significant cross-reactivity or interference between oxLDL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between oxLDL and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1?g/dl

Guinea Pig ELISA Kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This OXLDL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig OXLDL. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY OXLDL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OXLDL antibody and an OXLDL-HRP conjugate. The assay sample and buffer are incubated together with OXLDL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OXLDL concentration since OXLDL from samples and OXLDL-HRP conjugate compete for the anti-OXLDL antibody binding site. Since the number of sites is limited, as more sites are occupied by OXLDL from the sample, fewer sites are left to bind OXLDL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OXLDL concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470 USD

96-Strip-Wells

675