catalog number :
MBS725514
products type :
ELISA Kit
products full name :
Rat orphanin FQ/nociceptin ELISA Kit
products short name :
orphanin FQ/nociceptin
products name syn :
Rat orphanin FQ/nociceptin ELISA Kit; orphanin FQ/nociceptin; orphanin FQ/nociceptin (Rat)
products gene name :
OFQ/N
specificity :
This assay has high sensitivity and excellent specificity for detection of O-FQ. No significant cross-reactivity or interference between O-FQ and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between O-FQ and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This O-FQ ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat O-FQ. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Rat ELISA Kit
products description :
Principle of the Assay: O-FQ ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-O-FQ antibody and an O-FQ-HRP conjugate. The assay sample and buffer are incubated together with O-FQ-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the O-FQ concentration since O-FQ from samples and O-FQ-HRP conjugate compete for the anti-O-FQ antibody binding site. Since the number of sites is limited, as more sites are occupied by O-FQ from the sample, fewer sites are left to bind O-FQ-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The O-FQ concentration in each sample is interpolated from this standard curve.