ELISA/assay

Guinea Pig Epidermal growth factor ELISA Kit

MBS725510

48-Strip-Wells

470 USD

ELISA Kit

Guinea Pig Epidermal growth factor ELISA Kit

Epidermal growth factor

Guinea Pig Epidermal growth factor ELISA Kit; Epidermal growth factor; Epidermal growth factor (Guinea Pig)

epidermal growth factor

EGF

GuineaPig

This assay has high sensitivity and excellent specificity for detection of EGF. No significant cross-reactivity or interference between EGF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EGF and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Guinea Pig ELISA Kit

Intended Uses: This EGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea EGF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay EGF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-EGF antibody and an EGF-HRP conjugate. The assay sample and buffer are incubated together with EGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EGF concentration since EGF from samples and EGF-HRP conjugate compete for the anti-EGF antibody binding site. Since the number of sites is limited, as more sites are occupied by EGF from the sample, fewer sites are left to bind EGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EGF concentration in each sample is interpolated from this standard curve.

688072

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48-Strip-Wells

470 USD

96-Strip-Wells

675