catalog number :
MBS725482
products type :
ELISA Kit
products full name :
Human Calcitonin Gene Related Peptide ELISA Kit
products short name :
[Calcitonin Gene Related Peptide]
other names :
[calcitonin gene-related peptide isoform 3 preproprotein; Calcitonin gene-related peptide; calcitonin; calcitonin gene-related peptide; calcitonin-related polypeptide alpha; calcitonin/calcitonin-related polypeptide, alpha; calcitonin]
products gene name :
[CGRP]
other gene names :
[CALCA; CALCA; CALC; CGRP; CALC; CGRP]
uniprot entry name :
CALCA_CHICK
specificity :
This assay has high sensitivity and excellent specificity for detection of CGN1. No significant cross-reactivity or interference between CGN1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CGN1 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 ng/mL.
products categories :
Neurobiology
products description :
Intended Uses: This CGN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CGN1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CGN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CGN1 antibody and an CGN1-HRP conjugate. The assay sample and buffer are incubated together with CGN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CGN1 concentration since CGN1 from samples and CGN1-HRP conjugate compete for the anti-CGN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CGN1 from the sample, fewer sites are left to bind CGN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CGN1 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
NP_001258895.1
ncbi gb acc num :
NM_001271966.1
ncbi mol weight :
15,308 Da
uniprot summary :
Causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.
size1 :
48-Strip-Wells-(Competitive)
size2 :
48-Strip-Wells-(Sandwich)
size3 :
96-Strip-Wells-(Competitive)
size4 :
96-Strip-Wells-(Sandwich)
size5 :
5x96-Strip-Wells-(Competitive)
size6 :
5x96-Strip-Wells-(Sandwich)
size7 :
10x96-Strip-Wells-(Competitive)
size8 :
10x96-Strip-Wells-(Sandwich)