ELISA/assay

Rat heart fatty acid binding protein, h-FABP ELISA Kit

MBS725463

48-Strip-Wells

470 USD

ELISA Kit

Rat heart fatty acid binding protein, h-FABP ELISA Kit

heart fatty acid binding protein, h-FABP

Rat heart fatty acid binding protein ELISA Kit; Rat heart fatty acid binding protein, h-FABP ELISA Kit; heart fatty acid binding protein, h-FABP; heart fatty acid binding protein, h-FABP ELISA Kit (Rat)

heart fatty acid binding protein; Fatty acid-binding protein, heart; fatty acid-binding protein, heart; M-FABP; OTTHUMP00000003898; fatty acid binding protein 11; mammary-derived growth inhibitor; muscle fatty acid-binding protein; Fatty acid-binding protein 3, muscle; heart-type fatty acid-binding protein; fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor); Fatty acid-binding protein 3; Heart-type fatty acid-binding protein; H-FABP; Mammary-derived growth inhibitor; MDGI; Muscle fatty acid-binding protein

H-FABP

FABP3; FABP3; MDGI; FABP11; H-FABP; O-FABP; FABP11; MDGI

FABPH_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of HFABP. No significant cross-reactivity or interference between HFABP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HFABP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Rat ELISA Kit

Intended Uses: This HFABP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat HFABP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay HFABP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HFABP. Standards or samples are then added to the microtiter plate wells and HFABP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HFABP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HFABP are added to each well to "sandwich" the HFABP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HFABP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HFABP concentration in each sample is interpolated from this standard curve.

458862

P05413

14,858 Da

PPAR Signaling Pathway (83042); PPAR Signaling Pathway (450)

The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer. [provided by RefSeq]

Function: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. Subcellular location: Cytoplasm. Domain: Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. Sequence similarities: Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

48-Strip-Wells

470 USD

96-Strip-Wells

675