MyBioSource

ELISA/assay

Human Dystrophin ELISA Kit

MBS725446

96 Strip Wells

675 USD

MBS725446

ELISA Kit

Human Dystrophin ELISA Kit

Dystrophin

DMD; Dystrophin; dystrophin; mental retardation, X-linked 85; dystrophin; N/A

DMD

DMD; DMD; BMD; CMD3B; MRX85; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; N/A

DMD_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of DYS. No significant cross-reactivity or interference between DYS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DYS and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This DYS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DYS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Cell Biology

Principle of the assay: DYS ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for DYS. Standards or samples are then added to the microtiter plate wells and DYS if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of DYS present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for DYS are added to each well to “sandwich” the DYS immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain DYS and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DYS concentration in each sample is interpolated from this standard curve.

181599

AAA52316.1

57,953 Da

Arrhythmogenic Right Ventricular Cardiomyopathy Pathway 672454!!Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway 117293!!Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway 116129!!Dilated Cardiomyopathy Pathway 121494!!Dilated Cardiomyopathy Pathway 121285!!Extracellular Matrix Organization Pathway 576262!!Hypertrophic Cardiomyopathy (HCM) Pathway 114229!!Hypertrophic Cardiomyopathy (HCM) Pathway 106591!!Muscle Contraction Pathway 106261!!Non-integrin Membrane-ECM Interactions Pathway 833810

The dystrophin gene is the largest gene found in nature, measuring 2.4 Mb. The gene was identified through a positional cloning approach, targeted at the isolation of the gene responsible for Duchenne (DMD) and Becker (BMD) Muscular Dystrophies. DMD is a recessive, fatal, X-linked disorder occurring at a frequency of about 1 in 3,500 new-born males. BMD is a milder allelic form. In general, DMD patients carry mutations which cause premature translation termination (nonsense or frame shift mutations), while in BMD patients dystrophin is reduced either in molecular weight (derived from in-frame deletions) or in expression level. The dystrophin gene is highly complex, containing at least eight independent, tissue-specific promoters and two polyA-addition sites. Furthermore, dystrophin RNA is differentially spliced, producing a range of different transcripts, encoding a large set of protein isoforms. Dystrophin (as encoded by the Dp427 transcripts) is a large, rod-like cytoskeletal protein which is found at the inner surface of muscle fibers. Dystrophin is part of the dystrophin-glycoprotein complex (DGC), which bridges the inner cytoskeleton (F-actin) and the extra-cellular matrix. [provided by RefSeq, Jul 2008]

96 Strip Wells

675 USD