ELISA/assay

Rat Activated Leukocyte Cell Adhesion Molecule ELISA Kit

MBS725431

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Rat Activated Leukocyte Cell Adhesion Molecule ELISA Kit

Activated Leukocyte Cell Adhesion Molecule

alcam; CD166 antigen; CD166 antigen; activated leukocyte cell adhesion molecule; Activated leukocyte cell adhesion molecule; CD_antigen: CD166

ALCAM

ALCAM; ALCAM; MEMD; CD166; MEMD

CD166_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of ALAM. No significant cross-reactivity or interference between ALAM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ALAM and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Intended Uses: This ALAM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ALAM. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: ALAM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ALAM antibody and an ALAM-HRP conjugate. The assay sample and buffer are incubated together with ALAM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALAM concentration since ALAM from samples and ALAM-HRP conjugate compete for the anti-ALAM antibody binding site. Since the number of sites is limited, as more sites are occupied by ALAM from the sample, fewer sites are left to bind ALAM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALAM concentration in each sample is interpolated from this standard curve.

886258

AAB59499.1

33,878 Da

Axon Guidance Pathway (105688); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Developmental Biology Pathway (477129); L1CAM Interactions Pathway (161003)

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]

ALCAM: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Cell adhesion. Chromosomal Location of Human Ortholog: 3q13.1. Cellular Component: focal adhesion; cell soma; axon; integral to membrane; external side of plasma membrane. Molecular Function: protein binding; receptor binding. Biological Process: axon guidance; motor axon guidance; cell adhesion; signal transduction

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675