catalog number :
MBS7253705
products type :
ELISA Kit
products full name :
Fish Follicle Stimulating Hormone (FSH) ELISA Kit
products short name :
[Follicle Stimulating Hormone (FSH)]
products gene name :
[FSH]
specificity :
This assay has high sensitivity and excellent specificity for detection of free Sialic acid. No significant cross-reactivity or interference between free Sialic acid and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between free Sialic acid and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 nmol/mL.
products description :
Intended Uses: This free Sialic acid ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human free Sialic acid. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: free Sialic acid ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-free Sialic acid antibody and an free Sialic acid-HRP conjugate. The assay sample and buffer are incubated together with free Sialic acid-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the free Sialic acid concentration since free Sialic acid from samples and free Sialic acid-HRP conjugate compete for the anti-free Sialic acid antibody binding site. Since the number of sites is limited, as more sites are occupied by free Sialic acid from the sample, fewer sites are left to bind free Sialic acid-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The free Sialic acid concentration in each sample is interpolated from this standard curve.
size4 :
10x96-Strip-Wells