ELISA/assay

Mouse Myoblast determination protein 1 (MYOD1) ELISA Kit

MBS7253634

48-Strip-Wells

440 USD

ELISA Kit

Mouse Myoblast determination protein 1 (MYOD1) ELISA Kit

[Myoblast determination protein 1 (MYOD1)]

[myoblast determination protein 1; Myoblast determination protein 1; myoblast determination protein 1; myf-3; myogenic factor 3; class C basic helix-loop-helix protein 1; myogenic differentiation 1; Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3]

[MYOD1]

[MYOD1; MYOD1; PUM; MYF3; MYOD; bHLHc1; BHLHC1; MYF3; MYOD; bHLHc1; Myf-3]

MYOD1_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of MYO7A. No significant cross-reactivity or interference between MYO7A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MYO7A and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

Epigenetics and Nuclear Signaling

Intended Uses: This MYO7A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit MYO7A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: MYO7A ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MYO7A antibody and an MYO7A-HRP conjugate. The assay sample and buffer are incubated together with MYO7A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MYO7A concentration since MYO7A from samples and MYO7A-HRP conjugate compete for the anti-MYO7A antibody binding site. Since the number of sites is limited, as more sites are occupied by MYO7A from the sample, fewer sites are left to bind MYO7A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MYO7A concentration in each sample is interpolated from this standard curve.

23111009

NP_002469.2

NM_002478.4

34,501 Da

C-MYB Transcription Factor Network Pathway (138073); CDO In Myogenesis Pathway (160998); Developmental Biology Pathway (477129); Id Signaling Pathway (198871); Myogenesis Pathway (160997); Notch-mediated HES/HEY Network Pathway (169347); Regulation Of Nuclear SMAD2/3 Signaling Pathway (137963); Regulation Of Retinoblastoma Protein Pathway (137916); Signaling Events Mediated By HDAC Class III Pathway (137972); MiRs In Muscle Cell Differentiation Pathway (672449)

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]

MyoD: a transcription factor of the basic helix-loop-helix family and the myogenic factors subfamily. Involved in muscle differentiation. Induces fibroblasts to differentiate into myoblasts. It is involved in muscle cell differentiation, and is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis. Protein type: Transcription factor; RNA splicing; DNA-binding. Chromosomal Location of Human Ortholog: 11p15.4. Cellular Component: nucleoplasm; transcription factor complex; myofibril; nuclear chromatin; cytoplasm; nucleus. Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein heterodimerization activity; chromatin DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; transcription factor binding; nuclear hormone receptor binding. Biological Process: transcription from RNA polymerase II promoter; skeletal muscle development; muscle development; skeletal muscle fiber adaptation; positive regulation of skeletal muscle regeneration; regulation of RNA splicing; myotube differentiation involved in skeletal muscle regeneration; muscle cell fate commitment; myotube cell development; skeletal muscle fiber development; cellular response to starvation; protein amino acid phosphorylation; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; positive regulation of skeletal muscle fiber development; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; myoblast cell fate determination; regulation of alternative nuclear mRNA splicing, via spliceosome

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415