ELISA/assay

Human Glycogen Synthase Kinase 3 beta ELISA Kit

MBS7253535

48-Strip-Wells

440 USD

ELISA Kit

Human Glycogen Synthase Kinase 3 beta ELISA Kit

[Glycogen Synthase Kinase 3 beta]

[Human Glycogen Synthase Kinase 3 b ELISA Kit]

[Glycogen synthase kinase-3 beta; Glycogen synthase kinase-3 beta; glycogen synthase kinase-3 beta; GSK-3 beta; GSK3beta isoform; serine/threonine-protein kinase GSK3B; glycogen synthase kinase 3 beta; Serine/threonine-protein kinase GSK3B (EC:2.7.11.1)]

[GSK 3beta]

[GSK 3b]

[GSK3B; GSK3B; GSK-3 beta]

GSK3B_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of GSK3beta. No significant cross-reactivity or interference between GSK3beta and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GSK3beta and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 0.5-10ng/mL. Sensitivity: 0.1ng/mL

Intended Uses: This GSK3beta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GSK3beta. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: GSK3beta ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GSK3beta antibody and an GSK3beta -HRP conjugate. The assay sample and buffer are incubated together with GSK3beta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GSK3beta concentration since GSK3beta from samples and GSK3beta -HRP conjugate compete for the anti-GSK3beta antibody binding site. Since the number of sites is limited, as more sites are occupied by GSK3beta from the sample, fewer sites are left to bind GSK3beta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSK3beta concentration in each sample is interpolated from this standard curve.

20455502

P49841.2

48,034 Da

AKT Phosphorylates Targets In The Cytosol Pathway (106475); AMER1 Mutants Destabilize The Destruction Complex Pathway (1016198); APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway (1016193); APC Truncation Mutants Have Impaired AXIN Binding Pathway (1016194); AXIN Missense Mutants Destabilize The Destruction Complex Pathway (1016197); AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway (1016195); Adaptive Immune System Pathway (366160); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448)

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation. Protein type: EC 2.7.11.1; Protein kinase, CMGC; Kinase, protein; EC 2.7.11.26; Protein kinase, Ser/Thr (non-receptor); CMGC group; GSK family; GSK subfamily. Chromosomal Location of Human Ortholog: 3q13.3. Cellular Component: centrosome; dendritic spine; beta-catenin destruction complex; ribonucleoprotein complex; cytosol; lipid raft; nucleoplasm; growth cone; cell soma; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; nucleus. Molecular Function: integrin binding; protein serine/threonine kinase activity; ionotropic glutamate receptor binding; NF-kappaB binding; protein binding; p53 binding; ubiquitin protein ligase binding; tau-protein kinase activity; beta-catenin binding; kinase activity; protein kinase binding; tau protein binding; ATP binding. Biological Process: fat cell differentiation; glycogen metabolic process; circadian rhythm; axon guidance; negative regulation of MAP kinase activity; genetic imprinting; re-entry into mitotic cell cycle; positive regulation of protein binding; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; response to lithium ion; positive regulation of axon extension; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; positive regulation of protein export from nucleus; protein export from nucleus; negative regulation of protein binding; positive regulation of cell-matrix adhesion; myoblast fusion; ER overload response; negative regulation of glycogen biosynthetic process; epidermal growth factor receptor signaling pathway; response to drug; regulation of microtubule-based process; cell migration; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; hippocampus development; negative regulation of dendrite morphogenesis; negative regulation of NFAT protein import into nucleus; positive regulation of peptidyl-serine phosphorylation; negative regulation of neuron maturation; organ morphogenesis; peptidyl-serine phosphorylation; positive regulation of protein complex assembly; establishment of cell polarity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; innate immune response; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein complex assembly; regulation of neuronal synaptic plasticity; negative regulation of apoptosis

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415