ELISA/assay

Rat Ubiquitin-like modifier-activating enzyme ATG7 (ATG7) ELISA Kit

MBS7253427

48-Strip-Wells

470 USD

ELISA Kit

Rat Ubiquitin-like modifier-activating enzyme ATG7 (ATG7) ELISA Kit

Ubiquitin-like modifier-activating enzyme ATG7 (ATG7)

ubiquitin-like modifier-activating enzyme ATG7 isoform c; Ubiquitin-like modifier-activating enzyme ATG7; ubiquitin-like modifier-activating enzyme ATG7; hAGP7; autophagy-related protein 7; ATG12-activating enzyme E1 ATG7; ATG7 autophagy related 7 homolog; ubiquitin activating enzyme E1-like protein; ubiquitin-activating enzyme E1-like protein; autophagy related 7; ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; APG7-like; hAGP7; Ubiquitin-activating enzyme E1-like protein

ATG7

ATG7; ATG7; GSA7; APG7L; APG7-LIKE; APG7L; APG7-like; hAGP7

ATG7_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of ATG7. No significant cross-reactivity or interference between ATG7 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATG7 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This ATG7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ATG7. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: ATG7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATG7 antibody and an ATG7-HRP conjugate. The assay sample and buffer are incubated together with ATG7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATG7 concentration since ATG7 from samples and ATG7-HRP conjugate compete for the anti-ATG7 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATG7 from the sample, fewer sites are left to bind ATG7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATG7 concentration in each sample is interpolated from this standard curve.

222144229

NP_001138384.1

NM_001144912.1

68,618 Da

Adaptive Immune System Pathway (366160); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Immune System Pathway (106386); Regulation Of Autophagy Pathway (83058); Regulation Of Autophagy Pathway (468); Senescence And Autophagy Pathway (198780)

This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq, Jan 2009]

ATG7: Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3. Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. Belongs to the ATG7 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Autophagy; Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: 3p25.3. Cellular Component: cytoplasm; axoneme; cytosol. Molecular Function: protein binding; protein homodimerization activity; APG8 activating enzyme activity; APG12 activating enzyme activity; ubiquitin activating enzyme activity; transcription factor binding. Biological Process: regulation of protein ubiquitination; positive regulation of apoptosis; protein ubiquitination; pyramidal neuron development; adult walking behavior; post-embryonic development; protein transport; cardiac muscle cell development; protein amino acid lipidation; protein catabolic process; positive regulation of autophagy; amino acid metabolic process; central nervous system neuron axonogenesis; cerebellar Purkinje cell layer development; protein modification process; C-terminal protein lipidation; liver development; cellular response to starvation; neurological system process; cellular response to nitrogen starvation; protein modification by small protein conjugation; mitochondrion degradation; positive regulation of protein catabolic process; positive regulation of protein modification process; cerebral cortex development; positive regulation of macroautophagy; negative regulation of apoptosis

48-Strip-Wells

470 USD

96-Strip-Wells

675