ELISA/assay

Porcine C-X-C motif chemokine 2 (CXCL2) ELISA Kit

MBS7253248

48-Strip-Wells

470 USD

ELISA Kit

Porcine C-X-C motif chemokine 2 (CXCL2) ELISA Kit

C-X-C motif chemokine 2 (CXCL2)

C-X-C motif chemokine 2; C-X-C motif chemokine 2; C-X-C motif chemokine 2; gro-beta; MGSA beta; MIP2-alpha; GRO2 oncogene; growth-regulated protein beta; macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; chemokine (C-X-C motif) ligand 2; Growth-regulated protein beta; Gro-beta; Macrophage inflammatory protein 2-alpha; MIP2-alpha

CXCL2

CXCL2; CXCL2; GRO2; GROb; MIP2; MIP2A; SCYB2; MGSA-b; MIP-2a; CINC-2a; GRO2; GROB; MIP2A; SCYB2; Gro-beta; MIP2-alpha; HSF

CXCL2_HUMAN

Porcine

This assay has high sensitivity and excellent specificity for detection of CXCL-2. No significant cross-reactivity or interference between CXCL-2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CXCL-2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This CXCL-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine CXCL-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: CXCL-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CXCL-2 antibody and an CXCL-2-HRP conjugate. The assay sample and buffer are incubated together with CXCL-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CXCL-2 concentration since CXCL-2 from samples and CXCL-2-HRP conjugate compete for the anti-CXCL-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CXCL-2 from the sample, fewer sites are left to bind CXCL-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CXCL-2 concentration in each sample is interpolated from this standard curve.

4504155

NP_002080.1

NM_002089.3

11,389 Da

Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020)

This antimicrobial gene is part of a chemokine superfamily that encodes secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CXC subfamily, is expressed at sites of inflammation and may suppress hematopoietic progenitor cell proliferation. [provided by RefSeq, Sep 2014]

CXCL2: Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. Belongs to the intercrine alpha (chemokine CxC) family. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 4q21. Cellular Component: extracellular space; extracellular region. Molecular Function: protein binding; chemokine activity; CXCR chemokine receptor binding. Biological Process: G-protein coupled receptor protein signaling pathway; response to molecule of bacterial origin; immune response; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; inflammatory response; chemotaxis; regulation of cell proliferation

48-Strip-Wells

470 USD

96-Strip-Wells

675