ELISA/assay

Mouse Angiotensin II Converting Enzyme ELISA Kit

MBS725288

48-Strip-Wells

470 USD

ELISA Kit

Mouse Angiotensin II Converting Enzyme ELISA Kit

Angiotensin II Converting Enzyme

Mouse Angiotensin II Converting Enzyme ELISA Kit; Angiotensin II Converting Enzyme; Angiotensin II Converting Enzyme (Mouse)

angiotensin-converting enzyme 2; Angiotensin-converting enzyme 2; angiotensin-converting enzyme 2; OTTHUMP00000022963; metalloprotease MPROT15; ACE-related carboxypeptidase; angiotensin I converting enzyme 2; angiotensin-converting enzyme homolog; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; ACEH; Metalloprotease MPROT15

ACE II

ACE2; ACE2; ACEH; DKFZp434A014

ACE2_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of ACE2. No significant cross-reactivity or interference between ACE2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACE2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0pg/mL.

Mouse ELISA Kit

Intended Uses: This ACE2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ACE2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay ACE2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ACE2. Standards or samples are then added to the microtiter plate wells and ACE2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ACE2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ACE2 are added to each well to "sandwich" the ACE2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ACE2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACE2 concentration in each sample is interpolated from this standard curve.

11225609

NP_068576.1

NM_021804.2

Q9BYF1

92,463 Da

ACE Inhibitor Pathway (198763); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868); Renin-angiotensin System Pathway (83075); Renin-angiotensin System Pathway (486)

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq]

Function: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. Ref.1 Ref.2 Ref.14. Catalytic activity: Angiotensin II + H2O = angiotensin-1-7 + L-phenylalanine. Cofactor: Binds 1 zinc ion per subunit. Ref.13Binds 1 chloride ion per subunit. Ref.13. Enzyme regulation: Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. Ref.1 Ref.2 Ref.3 Ref.13. Subunit structure: Interacts with ITGB1. Interacts with SARS-CoV and HCoV-NL63 spike glycoprotein. Ref.11 Ref.14 Ref.17 Ref.20 Ref.22. Subcellular location: Processed angiotensin-converting enzyme 2: Secreted Ref.3. Cell membrane; Single-pass type I membrane protein Ref.3. Tissue specificity: Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system. Ref.1 Ref.2 Ref.3 Ref.12 Ref.16 Ref.19. Induction: Up-regulated in failing heart. Ref.1 Ref.2 Ref.3 Ref.13 Ref.15 Ref.19. Post-translational modification: N-glycosylation on Asn-90 may limit SARS infectivity. Sequence similarities: Belongs to the peptidase M2 family. Biophysicochemical propertiesKinetic parameters:KM=6.9 uM for angiotensin I Ref.13KM=2 uM for angiotensin IIKM=6.8 uM for apelin-13KM=5.5 uM for dynorphin-13pH dependence:Optimum pH is 6.5 in the presence of 1 M NaCl. Active from pH 6 to 9.

48-Strip-Wells

470 USD

96-Strip-Wells

675