ELISA/assay

Mouse Low-density lipoprotein receptor-related protein 1 ELISA Kit

MBS725254

48-Strip-Wells

470 USD

ELISA Kit

Mouse Low-density lipoprotein receptor-related protein 1 ELISA Kit

Low-density lipoprotein receptor-related protein 1

LRP1 protein; Prolow-density lipoprotein receptor-related protein 1; prolow-density lipoprotein receptor-related protein 1; LRP-1; type V tgf-beta receptor; apolipoprotein E receptor; alpha-2-macroglobulin receptor; TbetaR-V/LRP-1/IGFBP-3 receptor; low density lipoprotein receptor-related protein 1; Alpha-2-macroglobulin receptor; A2MR; Apolipoprotein E receptor; APOER; CD_antigen: CD91Cleaved into the following 3 chains:Low-density lipoprotein receptor-related protein 1 85 kDa subunit; LRP-85; Low-density lipoprotein receptor-related protein 1 515 kDa subunit; LRP-515; Low-density lipoprotein receptor-related protein 1 intracellular domain; LRPICD

LRP1

LRP1; LRP1; APR; LRP; A2MR; CD91; APOER; LRP1A; TGFBR5; IGFBP3R; A2MR; APR; LRP-1; A2MR; APOER; LRP-85; LRP-515; LRPICD

LRP1_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of LRP-1. No significant cross-reactivity or interference between LRP-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LRP-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0pg/mL.

Cell Biology

Intended Uses: This LRP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse LRP-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay LRP-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LRP-1. Standards or samples are then added to the microtiter plate wells and LRP-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LRP-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for LRP-1 are added to each well to "sandwich" the LRP-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LRP-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LRP-1 concentration in each sample is interpolated from this standard curve.

47940659

AAH72015.1

504,606 Da

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Malaria Pathway (152665); Malaria Pathway (152657); PDGFR-beta Signaling Pathway (138071); Retinoid Metabolism And Transport Pathway (187208)

The protein encoded by this gene is an endocytic receptor involved in several cellular processes, including intracellular signaling, lipid homeostasis, and clearance of apoptotic cells. In addition, the encoded protein is necessary for the A2M-mediated clearance of secreted amyloid precursor protein and beta-amyloid, the main component of amyloid plaques found in Alzheimer patients. Expression of this gene decreases with age and has been found to be lower than controls in brain tissue from Alzheimer patients. [provided by RefSeq, Jan 2010]

LRP1: a receptor involved in endocytosis, phagocytosis of apoptotic cells, and neural signaling. Modulates cellular events including APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling and neurotransmission. Involved in the plasma clearance of chylomicron remnants and activated alpha 2-macroglobulin, as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. Plays a fundamental role in cellular signaling pathways in the brain. Mediates neural NMDA gating via a complex of LRP1, PSD-95 and NMDAR. A type I membrane protein. After cleavage, the intracellular domain (LRPICD) is detected both in the cytoplasm and in the nucleus. Most abundant in liver, brain and lung. Protein type: Membrane protein, integral; Receptor, misc.; Cell surface; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 12q13.3. Cellular Component: focal adhesion; cell soma; clathrin-coated vesicle; lysosomal membrane; integral to plasma membrane; dendrite; cytoplasm; nucleolus; plasma membrane; coated pit; receptor complex; endosome. Molecular Function: protein binding; lipoprotein transporter activity; protease binding; protein complex binding; apolipoprotein binding; calcium ion binding; receptor activity. Biological Process: receptor-mediated endocytosis; cholesterol metabolic process; phototransduction, visible light; regulation of phospholipase A2 activity; negative regulation of Wnt receptor signaling pathway; lipoprotein metabolic process; negative regulation of smooth muscle cell migration; positive regulation of protein transport; cell proliferation; positive regulation of lipid transport; lipoprotein transport; regulation of actin cytoskeleton organization and biogenesis; protein kinase C activation; negative regulation of neuron apoptosis; retinoid metabolic process; regulation of cholesterol transport; apoptotic cell clearance; aging

48-Strip-Wells

470 USD

96-Strip-Wells

675