ELISA/assay

Mouse Protein Kinase Inhibitor Alpha ELISA Kit

MBS725250

48-Strip-Wells

470 USD

ELISA Kit

Mouse Protein Kinase Inhibitor Alpha ELISA Kit

Protein Kinase Inhibitor Alpha

PKIA; cAMP-dependent protein kinase inhibitor alpha; cAMP-dependent protein kinase inhibitor alpha; PKI-alpha; cAMP-dependent protein kinase inhibitor, muscle/brain isoform; protein kinase (cAMP-dependent, catalytic) inhibitor alpha; cAMP-dependent protein kinase inhibitor, muscle/brain isoform

PKIa

PKIA; PKIA; PRKACN1; PRKACN1; PKI-alpha

IPKA_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of PKIalpha. No significant cross-reactivity or interference between PKIalpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PKIalpha and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This PKIalpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PKIalpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: PKIalpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PKIalpha antibody and an PKIalpha-HRP conjugate. The assay sample and buffer are incubated together with PKIalpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PKIalpha concentration since PKIalpha from samples and PKIalpha-HRP conjugate compete for the anti-PKIalpha antibody binding site. Since the number of sites is limited, as more sites are occupied by PKIalpha from the sample, fewer sites are left to bind PKIalpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKIalpha concentration in each sample is interpolated from this standard curve.

48146221

CAG33333.1

P61925

7,989 Da

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Calcium Regulation In The Cardiac Cell Pathway (198906); Integrated Breast Cancer Pathway (219801); Myometrial Relaxation And Contraction Pathways (198759)

The protein encoded by this gene is a member of the cAMP-dependent protein kinase (PKA) inhibitor family. This protein was demonstrated to interact with and inhibit the activities of both C alpha and C beta catalytic subunits of the PKA. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

PKIA: Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains. Belongs to the PKI family. Protein type: Inhibitor. Chromosomal Location of Human Ortholog: 8q21.12. Cellular Component: cytoplasm; nucleus. Molecular Function: protein binding; cAMP-dependent protein kinase inhibitor activity. Biological Process: regulation of G2/M transition of mitotic cell cycle; negative regulation of protein import into nucleus; negative regulation of transcription from RNA polymerase II promoter

48-Strip-Wells

470 USD

96-Strip-Wells

675