ELISA/assay

Rat Stromal cell derived factor 1a ELISA Kit

MBS725246

48-Strip-Wells

470 USD

ELISA Kit

Rat Stromal cell derived factor 1a ELISA Kit

Stromal cell derived factor 1a

Rat Stromal cell derived factor 1a ELISA Kit; Stromal cell derived factor 1a; Stromal cell derived factor 1a (Rat)

stromal cell-derived factor 1 isoform alpha; CXCL12 protein; stromal cell-derived factor 1; OTTHUMP00000019491; OTTHUMP00000019492; OTTHUMP00000216979; OTTHUMP00000216980; intercrine reduced in hepatomas; pre-B cell growth-stimulating factor; chemokine (C-X-C motif) ligand 12

SDF-1a

CXCL12; CXCL12; IRH; PBSF; SDF1; TLSF; SDF1A; SDF1B; TPAR1; SCYB12

Q6ICW0_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of SDF-1alpha. No significant cross-reactivity or interference between SDF-1alpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SDF-1alpha and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This SDF-1alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat SDF-1alpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Rat ELISA Kit

Principle of the assay: SDF-1alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SDF-1alpha antibody and an SDF-1alpha-HRP conjugate. The assay sample and buffer are incubated together with SDF-1alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SDF-1alpha concentration since SDF-1alpha from samples and SDF-1alpha-HRP conjugate compete for the anti-SDF-1alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by SDF-1alpha from the sample, fewer sites are left to bind SDF-1alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDF-1alpha concentration in each sample is interpolated from this standard curve.

40316924

NP_954637.1

NM_199168.3

Q6ICW0

10,103 Da

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); CXCR4-mediated Signaling Events Pathway (137910); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); G Alpha (i) Signalling Events Pathway (119550)

This gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

CXCL12: Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to another C-X-C chemokine receptor CXCR7, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1- alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3- 67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and CXCR7, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T- cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of CXCR7 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Monomer or homodimer; in equilibrium. Dimer formation is induced by non acidic pH and the presence of multivalent anions, and by binding to CXCR4 or heparin. Monomeric form is required for full chemotactic activity and resistance to ischemia/reperfusion injury, whereas the dimeric form acts as a partial agonist of CXCR4, stimulating Ca2+ mobilization but with no chemotactic activity and instead acts as a selective antagonist that blocks chemotaxis induced by the monomeric form. Interacts with the N- terminus of CXCR7. Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen. Belongs to the intercrine alpha (chemokine CxC) family. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Cell development/differentiation; Chemokine; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 10q11.1. Cellular Component: extracellular space; extracellular region; external side of plasma membrane. Molecular Function: growth factor activity; chemokine receptor binding; chemokine activity; CXCR chemokine receptor binding; receptor binding. Biological Process: positive regulation of dopamine secretion; response to peptide hormone stimulus; positive regulation of cell adhesion; adult locomotory behavior; blood circulation; neuron migration; motor axon guidance; chemotaxis; signal transduction; induction of positive chemotaxis; germ cell development; T cell proliferation; response to radiation; germ cell migration; cell adhesion; organ regeneration; regulation of actin polymerization and/or depolymerization; response to virus; cellular calcium ion homeostasis; patterning of blood vessels; G-protein coupled receptor protein signaling pathway; response to heat; response to mechanical stimulus; ameboidal cell migration; positive regulation of axon extension involved in axon guidance; response to hypoxia; immune response; positive regulation of endothelial cell proliferation; telencephalon cell migration. Disease: Human Immunodeficiency Virus Type 1, Susceptibility To

48-Strip-Wells

470 USD

96-Strip-Wells

675