ELISA/assay

Human Thyroid Stimulating Hormone ELISA Kit

MBS725213

48-Strip-Wells

470 USD

ELISA Kit

Human Thyroid Stimulating Hormone ELISA Kit

Thyroid Stimulating Hormone

Human Thyroid Stimulating Hormone ELISA Kit; Thyroid Stimulating Hormone; Thyroid Stimulating Hormone (Human)

Tsh

TSH

Human

This assay has high sensitivity and excellent specificity for detection of TSH. No significant cross-reactivity or interference between TSH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TSH and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

Human ELISA Kit

Intended Uses: This TSH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TSH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: TSH ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TSH. Standards or samples are then added to the microtiter plate wells and TSH if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TSH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TSH are added to each well to "sandwich" the TSH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TSH and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TSH concentration in each sample is interpolated from this standard curve.

469236

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

48-Strip-Wells

470 USD

96-Strip-Wells

675