ELISA/assay

Rat Eosinophil peroxidase (EPX) ELISA Kit

MBS7251673

48-Strip-Wells

470 USD

ELISA Kit

Rat Eosinophil peroxidase (EPX) ELISA Kit

Eosinophil peroxidase (EPX)

eosinophil peroxidase; Eosinophil peroxidase; eosinophil peroxidase; eosinophil peroxidase

EPX

EPX; EPX; EPO; EPP; EPX-PEN; EPER; EPO; EPP; EPO

PERE_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of EPX. No significant cross-reactivity or interference between EPX and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EPX and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This EPX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat EPX. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: EPX ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-EPX antibody and an EPX-HRP conjugate. The assay sample and buffer are incubated together with EPX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EPX concentration since EPX from samples and EPX-HRP conjugate compete for the anti-EPX antibody binding site. Since the number of sites is limited, as more sites are occupied by EPX from the sample, fewer sites are left to bind EPX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EPX concentration in each sample is interpolated from this standard curve.

182146

AAA58458.1

81,040 Da

Asthma Pathway (83120); Asthma Pathway (532)

This gene is a member of the peroxidase gene family and is expressed in eosinophils. The encoded precursor protein is processed into covalently attached heavy and light chains to form the mature enzyme, which functions as an oxidant. The enzyme is released at sites of parasitic infection or allergen stimulation to mediate lysis of protozoa or parasitic worms. The gene is found in a cluster of three peroxidase genes at chromosome 17q23. Mutations in this gene result in eosinophil peroxidase deficiency. [provided by RefSeq, Sep 2009]

EPX: Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis. Defects in EPX are the cause of eosinophil peroxidase deficiency (EPD). EPD is an autosomal recessive defect where anomalous eosinophils are characterized by nuclear hypersegmentation, hypogranulation, and negative peroxidase and phospholipid staining. Belongs to the peroxidase family. XPO subfamily. Protein type: EC 1.11.1.7; Oxidoreductase. Chromosomal Location of Human Ortholog: 17q23.1. Molecular Function: peroxidase activity; metal ion binding; heme binding. Biological Process: positive regulation of interleukin-4 production; negative regulation of interleukin-10 production; hydrogen peroxide catabolic process; negative regulation of interleukin-5 production; response to oxidative stress; defense response to nematode. Disease: Eosinophil Peroxidase Deficiency

48-Strip-Wells

470 USD

96-Strip-Wells

675