Human Filaggrin ELISA Kit | MyBioSource MBS7251319 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Filaggrin ELISA Kit

catalog :

MBS7251319

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS7251319

products type :

ELISA Kit

products full name :

Human Filaggrin ELISA Kit

products short name :

Filaggrin

other names :

filaggrin; Filaggrin; filaggrin; epidermal filaggrin; filaggrin

products gene name :

Filaggrin

other gene names :

FLG; FLG; ATOD2

uniprot entry name :

FILA_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of FLG. No significant cross-reactivity or interference between FLG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FLG and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This FLG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FLG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

. Principle of the assay: FLG ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FLG. Standards or samples are then added to the microtiter plate wells and FLG if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FLG present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FLG are added to each well to "sandwich" the FLG immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FLG and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FLG concentration in each sample is interpolated from this standard curve.

ncbi gi num :

60097902

ncbi acc num :

NP_002007.1

ncbi gb acc num :

NM_002016.1

ncbi mol weight :

435,170 Da

ncbi pathways :

AhR Pathway (755436)

ncbi summary :

The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009]

uniprot summary :

FLG: Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. Defects in FLG are the cause of ichthyosis vulgaris (VI); also known as ichthyosis simplex. Ichthyosis vulgaris is the most common form of ichthyosis inherited as an autosomal dominant trait. It is characterized by palmar hyperlinearity, keratosis pilaris and a fine scale that is most prominent over the lower abdomen, arms, and legs. Ichthyosis vulgaris is characterized histologically by absent or reduced keratohyalin granules in the epidermis and mild hyperkeratosis. The disease can be associated with frequent asthma, eczema or hay fever. Defects in FLG are a cause of susceptibility to dermatitis atopic type 2 (ATOD2). Atopic dermatitis is a complex, inflammatory disease with multiple alleles at several loci thought to be involved in the pathogenesis. It commonly begins in infancy or early childhood and is characterized by a chronic relapsing form of skin inflammation, a disturbance of epidermal barrier function that culminates in dry skin, and IgE- mediated sensitization to food and environmental allergens. It is manifested by lichenification, excoriation, and crusting, mainly on the flexural surfaces of the elbow and knee. Belongs to the S100-fused protein family. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 1q21.3. Cellular Component: cytoplasmic membrane-bound vesicle; intermediate filament; nucleus. Molecular Function: protein binding; calcium ion binding; structural molecule activity. Biological Process: keratinocyte differentiation; multicellular organismal development. Disease: Ichthyosis Vulgaris; Dermatitis, Atopic, 2

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage