ELISA/assay

Rat Tau Protein ELISA Kit

MBS725098

48-Strip-Wells

470 USD

ELISA Kit

Rat Tau Protein ELISA Kit

Tau Protein

tau, isoform A; Microtubule-associated protein; CG45110 gene product from transcript CG45110-RH; CG45110-PA; CG45110-PB; CG45110-PC; CG45110-PE; CG45110-PF; CG45110-PG; CG45110-PH; CG45110-PI; CG45110-PJ; CG45110-PK; CG45110-PL; CG45110-PM; tau; tau-PA; tau-PB; tau-PC; tau-PE; tau-PF; tau-PG; tau-PH; tau-PI; tau-PJ; tau-PK; tau-PL; tau-PM

Tau

tau; tau; BcDNA:RE16764; CG12881; CG31057; CG31058; CG34294; CG45110; CG5606; d-tau; DmelCG45110; dtau; dTau; Tau; CG34294; Dmel_CG31057

Q9VB13_DROME

Rat

This assay has high sensitivity and excellent specificity for detection of TAU. No significant cross-reactivity or interference between TAU and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TAU and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL

Neurobiology

Intended Uses: This TAU ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat TAU. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: TAU ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TAU antibody and an TAU-HRP conjugate. The assay sample and buffer are incubated together with TAU-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TAU concentration since TAU from samples and TAU-HRP conjugate compete for the anti-TAU antibody binding site. Since the number of sites is limited, as more sites are occupied by TAU from the sample, fewer sites are left to bind TAU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TAU concentration in each sample is interpolated from this standard curve.

21357661

NP_651575.1

NM_143318.3

38,538 Da

Apoptosis Pathway (1005319); Apoptotic Cleavage Of Cellular Proteins Pathway (1005323); Apoptotic Execution Phase Pathway (1005322); Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway (1005324)

48-Strip-Wells

470 USD

96-Strip-Wells

675