product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Insulin-degrading enzyme ELISA Kit
catalog :
MBS725082
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
MyBioSource product webpage
citations: 2
Reference
Son S, Cha M, Choi H, Kang S, Choi H, Lee M, et al. Insulin-degrading enzyme secretion from astrocytes is mediated by an autophagy-based unconventional secretory pathway in Alzheimer disease. Autophagy. 2016;12:784-800 pubmed publisher
Son S, Kang S, Choi H, Mook Jung I. Statins induce insulin-degrading enzyme secretion from astrocytes via an autophagy-based unconventional secretory pathway. Mol Neurodegener. 2015;10:56 pubmed publisher
product information
catalog number :
MBS725082
products type :
ELISA Kit
products full name :
Mouse Insulin-degrading enzyme ELISA Kit
products short name :
Insulin-degrading enzyme
other names :
insulin degrading enzyme; insulin degrading enzyme
products gene name :
IDE
other gene names :
ide
reactivity :
Mouse
specificity :
This assay has high sensitivity and excellent specificity for detection of IDE. No significant cross-reactivity or interference between IDE and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IDE and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This IDE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse IDE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Immunology
products description :
Principle of the assay: IDE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IDE antibody and an IDE-HRP conjugate. The assay sample and buffer are incubated together with IDE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IDE concentration since IDE from samples and IDE-HRP conjugate compete for the anti-IDE antibody binding site. Since the number of sites is limited, as more sites are occupied by IDE from the sample, fewer sites are left to bind IDE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IDE concentration in each sample is interpolated from this standard curve.
ncbi gi num :
350535204
ncbi acc num :
NP_001233926.1
ncbi gb acc num :
NM_001246997.1
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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