ELISA/assay

Human Alkaline Phosphatase, Intestinal ELISA Kit

MBS725036

48-Strip-Wells

470 USD

ELISA Kit

Human Alkaline Phosphatase, Intestinal ELISA Kit

Alkaline Phosphatase, Intestinal

Alkaline phosphatase, intestinal; Intestinal-type alkaline phosphatase; intestinal-type alkaline phosphatase; Kasahara isozyme; glycerophosphatase; alkaline phosphomonoesterase; intestinal alkaline phosphatase; alkaline phosphatase, intestinal

ALPI

ALPI; ALPI; IAP; IAP; Intestinal alkaline phosphatase

PPBI_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of iALP. No significant cross-reactivity or interference between iALP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between iALP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This iALP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human iALP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: iALP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-iALP antibody and an iALP-HRP conjugate. The assay sample and buffer are incubated together with iALP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the iALP concentration since iALP from samples and iALP-HRP conjugate compete for the anti-iALP antibody binding site. Since the number of sites is limited, as more sites are occupied by iALP from the sample, fewer sites are left to bind iALP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The iALP concentration in each sample is interpolated from this standard curve.

124376142

AAI32679.1

56,812 Da

Folate Biosynthesis Pathway (83018); Folate Biosynthesis Pathway (404); Metabolic Pathways (132956)

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation. [provided by RefSeq, Jul 2008]

ALPI: intestinal alkaline phosphatase is one of four related alkaline phosphatases. The exact physiological function of alkaline phosphatases is not known. This protein is a GPI-anchored membrane bound glycosylated enzyme that is localized to fetal and adult intestines. Protein type: Motility/polarity/chemotaxis; Cofactor and Vitamin Metabolism - folate biosynthesis; EC 3.1.3.1; Phosphatase; Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 2q37.1. Cellular Component: plasma membrane; integral to membrane. Molecular Function: protease binding; zinc ion binding; alkaline phosphatase activity; magnesium ion binding. Biological Process: dephosphorylation

48-Strip-Wells

470 USD

96-Strip-Wells

675