ELISA/assay

Mouse alpha Synuclein ELISA Kit

MBS725030

48-Strip-Wells

470 USD

ELISA Kit

Mouse alpha Synuclein ELISA Kit

alpha Synuclein

Mouse a Synuclein ELISA Kit

alpha-synuclein; Alpha-synuclein; alpha-synuclein; non-A4 component of amyloid; non-A beta component of AD amyloid; synuclein, alpha; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP

alpha-SYN

a-SYN

Snca; Snca; NACP; alphaSYN; Syn; NACP

SYUA_MOUSE

Mouse

This assay has high sensitivity and excellent specificity for detection of SNCalpha. No significant cross-reactivity or interference between SNCalpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SNCalpha and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 ng/mL. Assay Type: Sandwich

Intended Uses: This SNCalpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SNCalpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

Principle of the Assay: SNCalpha ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SNCalpha. Standards or samples are then added to the microtiter plate wells and SNCalpha if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SNCalpha present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SNCalpha are added to each well to "sandwich" the SNCalpha immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SNCalpha and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SNCalpha concentration in each sample is interpolated from this standard curve.

109638747

NP_001035916.1

NM_001042451.2

12,345 Da

Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509); Amyloids Pathway (1000612); Disease Pathway (970606); EGFR1 Signaling Pathway (198278); Parkinson's Disease Pathway (83295)

SNCA: a member of the synuclein family. Abundantly expressed in the brain. Inhibits phospholipase D2 selectively. May integrate presynaptic signaling and membrane trafficking. Implicated in the pathogenesis of Parkinson s disease. A major component of amyloid plaques in the brains of patients with Alzheimer s disease. Two alternatively spliced isoforms transcripts have been identified. Protein type: Adaptor/scaffold. Cellular Component: Golgi apparatus; nuclear outer membrane; mitochondrion; terminal button; inclusion body; cytosol; synaptic vesicle; platelet alpha granule membrane; perinuclear region of cytoplasm; cytoplasm; synapse; cytoplasmic vesicle membrane; rough endoplasmic reticulum; intracellular membrane-bound organelle; cell; fibril; cell cortex; actin cytoskeleton; growth cone; cytoskeleton; axon; plasma membrane; ribosome; nerve terminal; nucleus. Molecular Function: protein domain specific binding; identical protein binding; zinc ion binding; histone binding; kinesin binding; metal ion binding; microtubule binding; ferrous iron binding; caspase inhibitor activity; beta-tubulin binding; magnesium ion binding; phosphoprotein binding; protein N-terminus binding; Hsp70 protein binding; oxidoreductase activity; calcium ion binding; arachidonic acid binding; dynein binding; protein binding; copper ion binding; phospholipase binding; enzyme binding; phospholipid binding; tau protein binding; alpha-tubulin binding. Biological Process: regulation of acyl-CoA biosynthetic process; adult locomotory behavior; regulation of neurotransmitter secretion; positive regulation of endocytosis; response to lipopolysaccharide; dopamine biosynthetic process; positive regulation of neurotransmitter secretion; dopamine metabolic process; response to magnesium ion; regulation of neuron apoptosis; synapse organization and biogenesis; negative regulation of neuron apoptosis; response to drug; negative regulation of dopamine metabolic process; synaptic vesicle endocytosis; negative regulation of transporter activity; regulation of neuronal synaptic plasticity; negative regulation of apoptosis; regulation of long-term neuronal synaptic plasticity; negative regulation of serotonin uptake; synaptic transmission, dopaminergic; negative regulation of norepinephrine uptake; mitochondrial membrane organization and biogenesis; microglial cell activation; negative regulation of caspase activity; negative regulation of monooxygenase activity; fatty acid metabolic process; regulation of dopamine secretion; positive regulation of synaptic transmission; negative regulation of histone acetylation; negative regulation of dopamine uptake; synaptic transmission; negative regulation of exocytosis; negative regulation of protein amino acid phosphorylation; behavioral response to cocaine; receptor internalization; phospholipid metabolic process; response to iron(II) ion; positive regulation of receptor recycling; caspase activation; neutral lipid metabolic process; protein destabilization; negative regulation of microtubule polymerization; regulation of glutamate secretion; regulation of macrophage activation; synaptic vesicle transport; positive regulation of peptidyl-serine phosphorylation; organelle ATP synthesis coupled electron transport; regulation of locomotion; positive regulation of release of sequestered calcium ion into cytosol; regulation of excitatory postsynaptic membrane potential

48-Strip-Wells

470 USD

96-Strip-Wells

675