ELISA/assay

Mouse antidiuretic hormone/vasopressin/arginine vasopressin ELISA Kit

MBS725028

48-Strip-Wells

470 USD

ELISA Kit

Mouse antidiuretic hormone/vasopressin/arginine vasopressin ELISA Kit

diuretic hormone/vasopressin/arginine vasopressin

Mouse antidiuretic hormone/vasopressin/arginine vasopressin ELISA Kit; antidiuretic hormone/vasopressin/arginine vasopressin; antidiuretic hormone/vasopressin/arginine vasopressin (Mouse)

Arginine vasopressin; Vasopressin-neurophysin 2-copeptin; vasopressin-neurophysin 2-copeptin; neurohypophyseal; prepro-AVP-NP II; antidiuretic hormone; vasopressin-neurophysin II-copeptin; prepro-arginine-vasopressin-neurophysin II; arginine vasopressin; AVP-NPIICleaved into the following 3 chains:Arg-vasopressin; Alternative name(s):; Arginine-vasopressinNeurophysin 2; Alternative name(s):; Neurophysin-II

ADH/VP/AVP

AVP; AVP; VP; ADH; ARVP; AVRP; AVP-NPII; ARVP; VP

NEU2_HUMAN

Mouse

Sensitivity: The sensitivity in this assay is 1.0 pg/mL. Specificity: This assay has high sensitivity and excellent specificity for detection of ADH. No significant cross-reactivity or interference between ADH and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

Mouse ELISA Kit

For Samples: Cell culture fluid & body fluid & tissue homogenate serum or blood plasma. Intended Uses: This ADH ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of ADH in the sample, this ADH ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus ADH concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of ADH utilizes a polyclonal anti-ADH antibody and an ADH-HRP conjugate. The assay sample and buffer are incubated together with ADH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADH concentration since ADH from samples and ADH-HRP conjugate compete for the anti-ADH antibody binding site. Since the number of sites is limited, as more sites are occupied by ADH from the sample, fewer sites are left to bind ADH-HRP conjugate. Standards of known ADH concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (O.D.) to the concentration of ADH. The ADH concentration in each sample is interpolated from this standard curve.

116497129

AAI26197.1

P01185

17,325 Da

Aquaporin-mediated Transport Pathway (187187); BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway (477138); Circadian Clock Pathway (187173); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Peptide Ligand-binding Receptors Pathway (106358)

This gene encodes a precursor protein consisting of arginine vasopressin and two associated proteins, neurophysin 2 and a glycopeptide, copeptin. Arginine vasopressin is a posterior pituitary hormone which is synthesized in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. Along with its carrier protein, neurophysin 2, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. The precursor is thought to be activated while it is being transported along the axon to the posterior pituitary. Arginine vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney, and also causes vasoconstriction of the peripheral vessels. This hormone can contract smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in this gene cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI). [provided by RefSeq, Mar 2010]

Function: Neurophysin 2 specifically binds vasopressin.Vasopressin has a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels. Subcellular location: Secreted. Involvement in disease: Diabetes insipidus, neurohypophyseal (NDI) [MIM:125700]: A disease characterized by persistent thirst, polydipsia and polyuria. Affected individuals are apparently normal at birth, but characteristically develop symptoms of vasopressin deficiency during childhood.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.4 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24 Ref.25 Ref.26 Ref.27 Ref.28 Ref.29 Ref.30 Ref.31 Ref.32 Ref.33 Ref.34 Ref.35 Ref.36. Sequence similarities: Belongs to the vasopressin/oxytocin family.

48-Strip-Wells

470 USD

96-Strip-Wells

675