catalog number :
MBS724962
products type :
ELISA Kit
products full name :
Human Prostaglandin E1 ELISA Kit
products short name :
Prostaglandin E1
products gene name :
PGE1
specificity :
This assay has high sensitivity and excellent specificity for detection of PGE1. No significant cross-reactivity or interference between PGE1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PGE1 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This PGE1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PGE1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
. Principle of the assay: PGE1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PGE1. Standards or samples are then added to the microtiter plate wells and PGE1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PGE1 present in the sample, a standardized prepaHumanion of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PGE1 are added to each well to "sandwich" the PGE1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substHumane solutions are added to each well. The enzyme (HRP) and substHumane are allowed to react over a short incubation period. Only those wells that contain PGE1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substHumane reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentHumanion of standards. The PGE1 concentHumanion in each sample is interpolated from this standard curve.
size1 :
48-Strip-Wells-(Competitive)
size2 :
48-Strip-Wells-(Sandwich)
size3 :
96-Strip-Wells-(Competitive)
size4 :
96-Strip-Wells-(Sandwich)