ELISA/assay

Human 14 3 3 protein beta/Alpha (YWHAB) ELISA Kit

MBS7249557

48-Strip-Wells

470 USD

ELISA Kit

Human 14 3 3 protein beta/Alpha (YWHAB) ELISA Kit

14 3 3 protein beta/Alpha (YWHAB)

Human 14 3 3 protein b/Alpha (YWHAB) ELISA Kit

14-3-3 protein beta/alpha; 14-3-3 protein beta/alpha; 14-3-3 protein beta/alpha; 14-3-3 alpha; protein 1054; epididymis secretory protein Li 1; brain protein 14-3-3, beta isoform; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1

YWHAB; YWHAB; HS1; GW128; YWHAA; KCIP-1; HEL-S-1; KCIP-1

1433B_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of YWHAB. No significant cross-reactivity or interference between YWHAB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between YWHAB and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Signal Transduction

Intended Uses This YWHAB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human YWHAB. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: YWHAB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-YWHAB antibody and an YWHAB-HRP conjugate. The assay sample and buffer are incubated together with YWHAB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the YWHAB concentration since YWHAB from samples and YWHAB-HRP conjugate compete for the anti-YWHAB antibody binding site. Since the number of sites is limited, as more sites are occupied by YWHAB from the sample, fewer sites are left to bind YWHAB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YWHAB concentration in each sample is interpolated from this standard curve.

21328448

NP_647539.1

NM_139323.3

27,850 Da

ARMS-mediated Activation Pathway (106466); Activation Of BAD And Translocation To Mitochondria Pathway (105659); Activation Of BH3-only Proteins Pathway (105658); Adaptive Immune System Pathway (366160); Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (105648); Axon Guidance Pathway (105688); Butyrate Response Factor 1 (BRF1) Destabilizes MRNA Pathway (187213); Calcium Regulation In The Cardiac Cell Pathway (198906); Cell Cycle Pathway (198811)

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

14-3-3 beta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 20q13.1. Cellular Component: focal adhesion; cytoplasmic vesicle membrane; transcriptional repressor complex; membrane; perinuclear region of cytoplasm; cytoplasm; melanosome; cytosol; nucleus. Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; enzyme binding; histone deacetylase binding; protein complex binding; phosphoprotein binding; transcription corepressor activity; phosphoserine binding. Biological Process: negative regulation of protein amino acid dephosphorylation; epidermal growth factor receptor signaling pathway; positive regulation of catalytic activity; axon guidance; fibroblast growth factor receptor signaling pathway; activation of MAPKK activity; nerve growth factor receptor signaling pathway; protein heterooligomerization; apoptosis; MAPKKK cascade; small GTPase mediated signal transduction; Ras protein signal transduction; cytoplasmic sequestering of protein; insulin receptor signaling pathway; innate immune response; gene expression; vascular endothelial growth factor receptor signaling pathway; negative regulation of transcription, DNA-dependent; protein targeting

48-Strip-Wells

470 USD

96-Strip-Wells

675