ELISA/assay

Mouse Antigen KI-67 (Ki-67) ELISA Kit

MBS7249525

48-Strip-Wells

470 USD

ELISA Kit

Mouse Antigen KI-67 (Ki-67) ELISA Kit

Antigen KI-67 (Ki-67)

Antigen KI-67; Antigen KI-67; antigen KI-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; antigen identified by monoclonal antibody Ki-67; marker of proliferation Ki-67

MKI67

MKI67; MKI67; KIA; MIB-; MIB-1; PPP1R105

KI67_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of Ki-67. No significant cross-reactivity or interference between Ki-67 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Ki-67 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Cancer

Intended Uses: This Ki-67 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse Ki-67. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay Ki-67 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Ki-67 antibody and an Ki-67-HRP conjugate. The assay sample and buffer are incubated together with Ki-67-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Ki-67 concentration since Ki-67 from samples and Ki-67-HRP conjugate compete for the anti-Ki-67 antibody binding site. Since the number of sites is limited, as more sites are occupied by Ki-67 from the sample, fewer sites are left to bind Ki-67-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Ki-67 concentration in each sample is interpolated from this standard curve.

118572663

P46013.2

319,444 Da

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers. Protein type: Cell cycle regulation; Nucleolus. Chromosomal Location of Human Ortholog: 10q26.2. Cellular Component: membrane; cytoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region. Molecular Function: protein C-terminus binding; protein binding; ATP binding. Biological Process: cell proliferation; meiosis

48-Strip-Wells

470 USD

96-Strip-Wells

675