ELISA/assay

Bovine Nerve Growth Factor ELISA Kit

MBS724951

48-Strip-Wells

470 USD

ELISA Kit

Bovine Nerve Growth Factor ELISA Kit

Nerve Growth Factor

NGF protein, partial; Beta-nerve growth factor; beta-nerve growth factor; nerve growth factor, beta subunit; nerve growth factor (beta polypeptide)

NGF

NGF; NGF; NGFB; HSAN5; Beta-NGF; NGFB; Beta-NGF

NGF_HUMAN

Bovine

This assay has high sensitivity and excellent specificity for detection of NGF. No significant cross-reactivity or interference between NGF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NGF and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This NGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine NGF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

Principle of the assay: NGF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NGF antibody and an NGF-HRP conjugate. The assay sample and buffer are incubated together with NGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NGF concentration since NGF from samples and NGF-HRP conjugate compete for the anti-NGF antibody binding site. Since the number of sites is limited, as more sites are occupied by NGF from the sample, fewer sites are left to bind NGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NGF concentration in each sample is interpolated from this standard curve.

34192369

AAH32517.2

26,959 Da

ARMS-mediated Activation Pathway (106466); Activation Of TRKA Receptors Pathway (106456); Apoptosis Pathway (83060); Apoptosis Pathway (470); Axonal Growth Stimulation Pathway (106453); BDNF Signaling Pathway (712093); Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (106443); Ceramide Signalling Pathway (106451); Frs2-mediated Activation Pathway (106465); Id Signaling Pathway (198871)

This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]

NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1p13.1. Cellular Component: extracellular space; Golgi lumen; cytoplasmic membrane-bound vesicle; extracellular region; endosome. Molecular Function: metalloendopeptidase inhibitor activity; nerve growth factor receptor binding; protein binding; growth factor activity; receptor signaling protein activity. Biological Process: response to nicotine; circadian rhythm; response to peptide hormone stimulus; activation of MAPKK activity; nerve growth factor receptor signaling pathway; adult locomotory behavior; positive regulation of apoptosis; regulation of neuron differentiation; positive regulation of axon extension; response to glucocorticoid stimulus; regulation of neurotransmitter secretion; response to lipopolysaccharide; regulation of axonogenesis; regulation of caspase activity; sensory perception of pain; negative regulation of cell cycle; nerve growth factor processing; response to radiation; cell-cell signaling; small GTPase mediated signal transduction; negative regulation of neuron apoptosis; inflammatory response; response to electrical stimulus; response to drug; positive regulation of neuron maturation; phosphoinositide-mediated signaling; positive regulation of protein amino acid autophosphorylation; positive regulation of nerve growth factor receptor signaling pathway; regulation of release of sequestered calcium ion into cytosol; memory; peripheral nervous system development; neuron apoptosis; induction of apoptosis via death domain receptors; phospholipase C activation; response to mechanical stimulus; response to ozone; Ras protein signal transduction; positive regulation of transcription factor activity; positive regulation of axonogenesis; neurite morphogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis. Disease: Neuropathy, Hereditary Sensory And Autonomic, Type V

48-Strip-Wells

470 USD

96-Strip-Wells

675